N9914
Polynucleotide phosphorylase from Synechocystis sp.
recombinant, expressed in E. coli
Sinonimo/i:
PNPase, Polyribonucleotide Nucleotidyltransferase
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100 μG
381,00 €
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Cambia visualizzazione
100 μG
381,00 €
About This Item
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Origine biologica
bacterial (Synechocystis sp.)
Livello qualitativo
Ricombinante
expressed in E. coli
Descrizione
Histidine tagged
Saggio
90% (SDS-PAGE)
Stato
solution
Attività specifica
≥500 units/mg protein
PM
85 kDa
tecniche
cell based assay: suitable
Compatibilità
suitable for molecular biology
applicazioni
cell analysis
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
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Descrizione generale
Polynuclotide phosphorlyase in spinach chloroplasts acts as a exonuclease and a poly(A) polymerase. [1]
Applicazioni
Azioni biochim/fisiol
Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3′ to 5′ exoribonuclease activity and a 3′-terminal oligonucleotide polymerase activity.
Polynucleotide phosphorylase localizes to the intermembrane space of mitochondria and has a critical function in regulating mitochondrial homeostasis in human cells. [4]
Definizione di unità
One unit will polymerize 1.0 μmole of ADP, releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37 °C.
Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, 60 mM KCl, 20% (w/v) Glycerol
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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