Passa al contenuto
Merck
Tutte le immagini(2)

Documenti fondamentali

Visualizza tutta la documentazione

MAK008

Sigma-Aldrich

Kit per saggio dell′idrossiprolina

sufficient for 100 colorimetric tests

Sinonimo/i:

Kit per il dosaggio di idrossiprolina

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(2)

About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 100 colorimetric tests

Metodo di rivelazione

colorimetric

Malattie correlate

dermatological diseases

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Descrizione generale

L′idrossiprolina (4-idrossiprolina) è un aminoacido[1] non proteinogeno formato dall′idrossilazione post-traduzionale della prolina. L′idrossiprolina è un componente di rilievo del collagene, in cui agisce da stabilizzatore della struttura a elica.[2] Essendo in grande misura confinata nel collagene, è possibile misurare i livelli di idrossiprolina come indicatori del contenuto in collagene. Le condizioni che aumentano il turnover del collagene possono far aumentare i livelli di idrossiprolina nel siero e nelle urine. I livelli di idrossiprolina nel siero e nelle urine possono essere usati come marcatori del riassorbimento osseo.[3][4]

Applicazioni

Il kit del saggio dell′idrossiprolina è stato usato per determinare la concentrazione di idrossiprolina e collagene.[5][6][7]

Compatibilità

Adatto al rilevamento dell′idrossiprolina in supernatanti di colture cellulari e tissutali, urina, plasma, siero e altri campioni biologici.

Principio

Nel kit del saggio dell′idrossiprolina, la concentrazione dell′idrossiprolina si determina per mezzo della reazione dell′idrossiprolina ossidata con la 4-(dimetilamino)benzaldeide (DMAB), che dà luogo a un prodotto colorimetrico (560 nm), proporzionale all′idrossiprolina presente.

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Avvertenze

Danger

Classi di pericolo

Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Ox. Liq. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT RE 2 - STOT SE 3

Organi bersaglio

Central nervous system, Thyroid

Rischi supp

EUH031

Codice della classe di stoccaggio

5.1B - Oxidizing hazardous materials

Punto d’infiammabilità (°F)

64.4 °F

Punto d’infiammabilità (°C)

18 °C

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
8L15K05550
8L14K05550
8J06K05550
7M14K05550
8H15K05550

Non trovi la versione di tuo interesse?

Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.

Cerca un COA

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Urinary biochemical markers for bone resorption during the menstrual cycle.
Gorai I, et al.
Calcified Tissue International, 57(2), 100-104 (1995)
Lodish H, et al.
Molecular Cell Biology. 4th edition. (2000)
Chunming Jiang et al.
BioMed research international, 2015, 867140-867140 (2016-02-18)
Acute kidney injury (AKI) is associated with an increased risk of developing advanced chronic kidney disease (CKD). Yet, effective interventions to prevent this conversion are unavailable for clinical practice. In this study, we examined the beneficial effects of Tanshinone IIA
Proline and hydroxyproline metabolism: implications for animal and human nutrition.
Wu G, et al.
Amino Acids, 40(4), 1053-1063 (2011)
M K North et al.
Meat science, 110, 145-152 (2015-08-01)
This study aimed to determine the optimum ageing period for vacuum-packed springbok (Antidorcas marsupialis) Longissimus thoracis et lumborum (LTL) and Biceps femoris (BF) muscle stored at 5.4 ± 1.0°C. Portions of muscle from seven male and six female springbok were
Visualizza tutti i documenti correlati

Domande

1–10 di 13 domande  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Utile?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Utile?

  3. Where can I find the protocol for the hydroxyproline assay ?

    1 risposta

    1. Please use the links below to access the protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/104/862/mak008pis-ms.pdf

      Available datasheets and protocols are typically located in the 'DOCUMENTATION' section under the 'More Documents' tab. Please see the link below to review this section of the product page:
      https://www.sigmaaldrich.com/product/sigma/mak008#product-documentation

      Utile?

  4. Can this kit be used to quantify 3D collagen scaffolds or for coatings on scaffolds? Has it been done?

    1 risposta

    1. This type of sample has not been tested with this assay kit. It is suggested to consult the scientific literature to determine if the specific sample type has been reported for this type of usage.

      Utile?

  5. 1. Are heart samples that have been flash frozen suitable for use with MAK008-1KT?

    2. The manual mentions 10 mg of tissue, but does it need to be an exact measurement? Is it challenging to accurately weigh the frozen tissue?

    1 risposta

    1. 1. Flash frozen tissue can be used with the kit. The recommended approach is to homogenize the tissue with room temperature water. Allow the tissue to thaw slightly by leaving it in the water for a few seconds before homogenization.

      2. The kit is optimized for use with 10 mg of tissue homogenate supernatant. Slight variations below or above 10 mg will not affect the assay significantly. However, it is advisable to avoid large differences in the weight or mass of the tissue.

      Utile?

  6. Is it possible to carry out the 90-minute incubation at 60 °C, as mentioned in the protocol after adding the DMAB Reagent, using a hot plate? and, should the plate be covered during this incubation step?

    1 risposta

    1. Yes, it is possible to perform the incubation on a hot plate. It is recommended to cover the plate during this process.

      Utile?

  7. Protocol recommends 10 mg tissue or cells for sample preparation. Is there a minimum quantity of cells required for this kit?

    1 risposta

    1. It is recommended to start with 1-2 million cells per sample. However, determining a specific minimum amount is challenging because the level of hydroxyproline can vary significantly between different sample types.

      Utile?

  8. Is this kit suitable for tissue lysate samples?

    1 risposta

    1. Yes, this kit is suitable for tissue lysate samples.

      Utile?

  9. What is the expiration date of the product?

    1 risposta

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Utile?

  10. I want to study collagen production in the MRC-5 cell line under different experimental conditions, so I was wondering if I can use this assay kit to study the same?

    1 risposta

    1. Yes, this kit is suitable for hydroxyproline detection, a major component of collagen. This kit is suitable for use in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

      Utile?

1–10 di 13 domande  

Recensioni

Nessuna valutazione

Filtri attivi

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.