I8640

IgG

technical grade, ≥80% (SDS-PAGE), buffered aqueous solution

• Sinonimo/i: IgG umana
• Numero MDL: MFCD00163923
• Codice UNSPSC: 12352203
• NACRES: NA.46

Proprietà

• Coniugato: unconjugated
• Livello qualitativo: 200
• Grado: technical grade
• Saggio: ≥80% (SDS-PAGE)
• Stato: buffered aqueous solution
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Human IgG antibodies are primarily responsible for the facilitation of humoral immune responses such as complement fixation, placental transport, and phagocytosis among many others[1]. IgG from human serum can be used as an economical alternative for reagent grade immunoglobulins where high purity is not essential. It can also be used in immunoelectrophoresis. The product is reactive in humans.

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections[2]
Human IgG is purified from normal human serum by fractionation.

Applicazioni

Purified human IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Technical grade human IgG may be used as an economical alternative to the reagent grade immunoglobulins, in applications where high purity is not required. IgG from human serum was used as ELISA standard in various studies.[3][4][5] A concentration range of 200 μg/ml to 1 mg/ml of human IgG was used for blocking in flow cytometry.[6][7][8]

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)