I4144

Interleukin-3 from mouse

IL-3, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Sinonimo/i: IL-3, mIL-3, Interleukin-3 from mouse
• Numero MDL: MFCD00164817
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Ricombinante: expressed in E. coli
• Saggio: ≥98% (SDS-PAGE)
• Forma fisica: lyophilized powder
• Potenza: 0.01-0.1 ng/mL ED₅₀/EC₅₀
• Qualità: endotoxin tested
• PM: 15.1 kDa
• Confezionamento: pkg of 10 and 50 μg
• tecniche: cell culture | mammalian: suitable
• Impurezze: ≤1.000 EU/μg
• Colore: white to yellow
• N° accesso UniProt: P01586
• Temperatura di conservazione: −20°C
• Informazioni sul gene: mouse ... Il3(16187)

Descrizione

Descrizione generale

Interleukin-3 is a hematopoietic growth factor, which stimulates basophil development and triggers histamine, IL-4 and IL-6 release. It improves monocyte and macrophage activation and phagocytosis, enhances the secretory function of eosinophils. Interleukin-3 promotes endothelial cell proliferation and migration.

Applicazioni

Interleukin-3 from mouse has been used for the determination of 5-lipoxygenase product leukotriene C4 (LTC4) formation. It has also been used as a component of RPMI 1640 medium for cell transfection.

Azioni biochim/fisiol

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

Stato fisico

Lyophilized with 0.5 mg of bovine serum albumin. Sterile filtered through a 0.2 μm filter.

Risultati analitici

The biological activity is measured by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)