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H4272

Sigma-Aldrich

Ialuronidasi

Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid

Sinonimo/i:

Ialuronato 4-glicanoidrolasi, Ialuronoglucosaminidasi

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About This Item

Numero CAS:
37326-33-3
Classificazione EC (Enzyme Commission):
3.2.1.35 (BRENDA, IUBMB)
Numero CE:
253-464-3
Numero MDL:
MFCD00131351
Codice UNSPSC:
12352204
NACRES:
NA.75

Sterilità

aseptically processed

Livello qualitativo

200

Tipo

Type IV-S

Forma fisica

powder

Attività specifica

750-3000 units/mg solid

PM

~55 kDa (four subunits of 14 kDa each)

Composizione

Protein, 80-105% biuret

tecniche

cell culture | embryo: suitable

Temperatura di conservazione

−20°C

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Categorie correlate

Applicazioni

Hyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass in the isolation of mouse embryos or from newly ovulated eggs, when preparing the eggs for in vitro culture.

Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded.

Azioni biochim/fisiol

La ialuronidasi degrada lo ialuronano ed è stato scoperto che è regolata in maniera inappropriata durante la progressione del cancro.
Questi enzimi scindono casualmente i legami glicosidici della β-N-acetilesosammina-[1→4] nell′acido ialuronico, nella condroitina e nei condroitina solfati.

Definizione di unità

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Nota sulla preparazione

Reconstitute 1 vial of hyaluronidase with 3 ml of M2 Medium (Product Nos. M 7167 or M 5910) or deionized water to obtain a 10 mg/ml stock solution. Dilute stock solution with M2 Medium to obtain a final working concentration of approximately 300 μg/ml.

Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Olio minerale

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Collagenasi

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Olio minerale

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Mammalian histone methyltransferase G9a (also called EHMT2) deposits H3K9me2 on chromatin and is essential for postimplantation development. However, its role in oogenesis and preimplantation development remains poorly understood. We show that H3K9me2-enriched chromatin domains in mouse oocytes are generally depleted
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Articoli

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Protocolli

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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