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GE29-0485-81

HiTrap® Protein G HP

Cytiva 29-0485-81, pack of 1 mL

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About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

Confezionamento

pack of 1 mL

Produttore/marchio commerciale

Cytiva 29-0485-81

Disponibilità

not available in North America

Condizioni di stoccaggio

(20% Ehtanol)
 (20% Ethanol)

Parametri

flow rate
42 psi

Dimensioni del letto

7 mm × 25 mm

Volume del letto

1 mL

Diam. int. colonna

7 mm

Matrice

highly cross-linked 6% agarose

Dimens. media delle particelle

34 μm

cleaning

2-9

working range

3-9

Capacità

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
 25 mg binding capacity(human IgG/column)

Temperatura di conservazione

2-8°C

Descrizione generale

HiTrap Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Caratteristiche e vantaggi

High binding capacity of 25 mg IgG/ml medium and fast binding kinetics.
34 μm bead size for high performance, high-resolution purifications.
Binds broad range of different species and subclasses of IgG.
Simple and proven method giving reproducible results.
Designed for use with syringe, peristalic pump, or chromatography system.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

HiTrap is a registered trademark of Cytiva

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

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This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

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General instructions for affinity purification using HiTrap columns from Cytiva.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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