E4906
Enterokinase from bovine intestine
BioUltra, recombinant, expressed in E. coli, ≥20 units/mg protein, ≥95% (SDS-PAGE)
Sinonimo/i:
Enteropeptidase
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Prodotti consigliati
Ricombinante
expressed in E. coli
Nome Commerciale
BioUltra
Saggio
≥95% (SDS-PAGE)
Forma fisica
solution
Attività specifica
≥20 units/mg protein
PM
150 kDa
Confezionamento
vial of ~0.2 unit
Concentrazione
≥0.1 mg/mL
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
Applicazioni
Enterokinase from bovine intestine has been used in a study to assess duodenase as a potential activator of cascade digestive proteases. Enterokinase from bovine intestine has also been used in a study to investigate an inhibitor of enteropeptidases and trypsin from the bovine duodenum.
The enzyme from Sigma has been used to compare the specific activity with that of purified, recombinant bovine enterokinase (light chain) overexpressed in Escherichia coli.
Typical conditions for fusion protein cleavage:
Adjust the concentration of the fusion protein to 1.5 mg/ml and a pH between 7.0-8.0 with 500 mM Tris-HCl, pH 8.0, 2.0 mM CaCl2, and 1% Tween® 20
Add enterokinase to fusion protein solution at a ratio of ~ 0.02 units per 1 mg fusion protein and mix
Incubate reaction mixture at ~25 °C for 16 hours
Adjust the concentration of the fusion protein to 1.5 mg/ml and a pH between 7.0-8.0 with 500 mM Tris-HCl, pH 8.0, 2.0 mM CaCl2, and 1% Tween® 20
Add enterokinase to fusion protein solution at a ratio of ~ 0.02 units per 1 mg fusion protein and mix
Incubate reaction mixture at ~25 °C for 16 hours
Enterokinase is a member of the S1 peptidase family. In vivo, it is responsble for the proteolytic activation of trypsin from trypsinogen. Enterokinase is used for site specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags.
Azioni biochim/fisiol
Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer, wherein, the light and the heavy chains are linked by two disulfide bridges. Native enterokinase is composed of an 800 amino acid heavy chain and a 235 amino acid light chain. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. This cleavage site is incorporated into the FLAG tag. The FLAG® protein expression system is based on the fusion of the 8 amino acid FLAG tag to the recombinant protein of choice. Cleavage by enterokinase removes the FLAG tag from the fusion protein. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.
Proprietà fisiche
28 kDa light chain form
Definizione di unità
One unit will produce 1.0 nanomole of trypsin from trypsinogen per min at pH 5.6 at 25 °C.
Stato fisico
supplied as a solution in 20 mM Tris-HCl, 200 mM NaCl, and 50% glycerol
Note legali
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
X Zheng et al.
The Journal of biological chemistry, 274(3), 1596-1605 (1999-01-09)
Enteropeptidase is a heterodimeric type II membrane protein of the brush border of duodenal enterocytes. In this location, enteropeptidase cleaves and activates trypsinogen, thereby initiating the activation of other intestinal digestive enzymes. Recombinant bovine enteropeptidase was sorted directly to the
A G Mikhaĭlova et al.
Voprosy meditsinskoi khimii, 44(4), 338-346 (1998-12-10)
Enteropeptidase inhibitor (DI) was isolated from bovine duodenum during purification of this enzyme. DI was purified by affinity chromatography on immobilised trypsin. DI preparations contain two main components: DI-9 (9 kD) and DI-20 (20 kD). The N-terminal amino acid sequence
T S Zamolodchikova et al.
Bioorganicheskaia khimiia, 24(4), 300-305 (1998-06-05)
The substrate specificity of duodenase from bovine duodenum mucosa to synthetic and natural polypeptides was studied. Amino acid residues preferential for duodenase in the P1 and P2 positions of the substrate were determined. It was shown that the enzyme is
Haidong Tan et al.
Protein expression and purification, 56(1), 40-47 (2007-08-21)
The nucleotide sequence encoding bovine enterokinase light chain (EK) from Chinese northern yellow bovine was isolated. Two single-nucleotide mutations, namely, C245G and A528T were identified. The gene encoding the Pro82Arg/Glu176Asp variant of known bovine EK was fused with glutathione S-transferase
Yutetsu Kuruma et al.
Nature protocols, 10(9), 1328-1344 (2015-08-14)
Cell-free gene expression systems are biotechnological tools for the in vitro production of proteins of interest. The addition of membrane vesicles (liposomes) enables the production of membrane proteins, including those in large-molecular-weight complexes, such as the SecYEG translocon or ATP
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.