D7876
Diamine Oxidase from porcine kidney
≥0.05 unit/mg solid
Sinonimo/i:
Amine:oxygen oxidoreductase (deaminating) (pyridoxal-containing)
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250 MG
96,10 €
1 G
282,00 €
5 G
1.080,00 €
10 G
1.700,00 €
Scegli un formato
Cambia visualizzazione
250 MG
96,10 €
1 G
282,00 €
5 G
1.080,00 €
10 G
1.700,00 €
About This Item
Prodotti consigliati
Origine biologica
Porcine kidney
Stato
solid
Attività specifica
≥0.05 unit/mg solid
PM
170 kDa
Solubilità
100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL
Attività estranea
monoamine oxidase (benzylamine substrate) ≤1%
Temperatura di conservazione
−20°C
Descrizione generale
Diamine oxidase from porcine kidney is a homodimer consisting of two equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper.[1] The molecular mass of the enzyme is found to be 170 kDa.[2] The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A.[3] Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.[1]
Applicazioni
Diamine Oxidase from porcine kidney has been used in the construction of histamine biosensor.[4]
Diamine oxidase from porcine kidney has been used in a study to investigate a luminescence-based test for determining ornithine decarboxylase activity. Diamine oxidase from porcine kidney has also been used in a study to investigate N-linked oligosaccharide structures in diamine oxidase.
Azioni biochim/fisiol
Diamine Oxidase catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide.[5] The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen metabolism.[6] Diamine oxidase is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide.[7]
Definizione di unità
One unit will oxidize 1.0 μmole of putrescine per hr at pH 7.2 at 37 °C.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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I clienti hanno visto anche
Diamine oxidase and catalase are expressed in the same cells but are present in different subcellular compartments in porcine kidney.
H G Schwelberger et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 48 Suppl 1, S81-S82 (1999-06-01)
Y Huang et al.
Carbohydrate research, 323(1-4), 111-125 (2000-04-27)
Structures of the N-linked glycans released from porcine kidney diamine oxidase (DAO) were characterized utilizing various analytical techniques, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS), high-performance capillary electrophoresis (HPCE), and high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The
A Rinaldi et al.
Preparative biochemistry, 12(1), 11-28 (1982-01-01)
Several methods for the isolation of apparently homogeneous pig kidney diamine oxidase have been reported in recent years (1-7), but these procedures allow to obtain only little amounts of material making very difficult the study of the molecular properties of
A PRELIMINARY INVESTIGATION ON A HISTAMINE BIOSENSOR CONSTRUCTED FROM DIAMINE OXIDASE IMMOBILISED ONTO AN OXYGEN PROBE
The Enzymes (1970)
HISTAMINE BIOSENSOR: A REVIEW
NorazlinaOthman F, et al.
The Malaysian Journal of Analytical Sciences (2006)
Protocolli
To standardize a procedure for the enzymatic assay of Diamine Oxidase.
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