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Merck

D6063

REDTaq® SuperPak™ DNA Polymerase

Taq for routine PCR with inert dye; with 10X buffer & dNTP mix

Sinonimo/i:

DNA polymerase with dNTP, DNA polymerase with loading dye, Taq DNA polymerase

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Informazioni su questo articolo

NACRES:
NA.55
UNSPSC Code:
41106300
Concentration:
1 unit/μL

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Quality Level

200

form

liquid

usage

sufficient for 250 reactions

feature

dNTPs included, hotstart: no

concentration

1 unit/μL

technique(s)

PCR: suitable

color

red

input

purified DNA

suitability

suitable for PCR

shipped in

wet ice

storage temp.

−20°C

Categorie correlate

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Questo articolo
D8187D4309D2812
REDTaq® SuperPak™ DNA Polymerase Taq for routine PCR with inert dye; with 10X buffer & dNTP mix

Sigma-Aldrich

D6063

REDTaq® SuperPak™ DNA Polymerase

JumpStart™ REDTaq® DNA Polymerase Hot-start Taq enzyme with inert dye, 10X buffer included

Sigma-Aldrich

D8187

JumpStart™ REDTaq® DNA Polymerase

REDTaq® DNA Polymerase Taq for routine PCR with inert dye, 10X buffer included

Sigma-Aldrich

D4309

REDTaq® DNA Polymerase

REDTaq® Genomic DNA Polymerase without MgCl2

Sigma-Aldrich

D2812

REDTaq® Genomic DNA Polymerase

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

form

liquid

form

liquid

form

liquid

form

liquid

concentration

1 unit/μL

concentration

1 unit/μL

concentration

1 unit/μL

concentration

1 unit/μL

suitability

suitable for PCR

suitability

suitable for PCR

suitability

suitable for PCR

suitability

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

REDTaq® SuperPak™ DNA Polymerase is Sigma′s Taq DNA Polymerase mixed with an inert red dye. REDTaq® SuperPak™ DNA Polymerase is a convenient package, that includes all the necessary components for a PCR reaction except primers, DNA template and water. It consists of Sigma′s high quality REDTaq DNA polymerase, 10 mM ultrapure deoxynucleotide mix and 10´ PCR reaction buffer. The dye provides quick recognition of reactions to which enzyme has been added as well as visual confirmation of complete mixing.

Application

REDTaq® SuperPak™ DNA Polymerase has been used in:
  • reverse transcriptase-polymerase chain reaction (RT-PCR)[1]
  • nested PCR[2]
  • routine PCR amplification[3]

Features and Benefits

  • Contains inert dye that enables visual confirmation of enzyme addition and direct loading onto agarose gel without the need for an additional loading dye
  • Suitable for nested PCR
  • Contains ultrapure 10mM dNTP mix in a separate vial

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
SuperPak is a trademark of Sigma-Aldrich Co. LLC

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 includedSDS
  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology ReagentSDS

related product

N° Catalogo
Descrizione
Determinazione del prezzo

Classe di stoccaggio

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 2

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
SLCJ5011
SLCH8736
SLCD2747
SLBX4809
SLBX4808

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D Alvira et al.
Neuroscience, 147(3), 746-756 (2007-06-23)
The mechanism involved in neuronal apoptosis is largely unknown. Studies performed on neuronal cell cultures provide information about the pathways which orchestrate the process of neuronal loss and potential drugs for the treatment of neurological disorders. In the present study
Vishnu Chintalgattu et al.
Journal of molecular and cellular cardiology, 35(3), 277-286 (2003-04-05)
Vascular endothelial growth factor (VEGF), produced predominantly by endothelial cells, is involved in angiogenesis and mitogenesis. Myofibroblasts (myoFb) are phenotypically transformed fibroblast-like cells found at the site of myocardial infarction. Since myoFb play a role in tissue repair/remodeling at the
M Yeste-Velasco et al.
Neuropharmacology, 53(2), 295-307 (2007-07-07)
Recent studies have demonstrated that neuronal reentry in the cell cycle and specifically the expression of the transcription factor E2F-1, constitutes a pathway that may be involved in neuronal apoptosis after serum and potassium withdrawal. Other enzymes such as glycogen
M Yeste-Velasco et al.
Neuroscience, 159(3), 1135-1147 (2009-04-10)
Increasing evidence implicates the c-Jun NH(2)-terminal kinase (JNK) pathway in the regulation of apoptosis in neurodegenerative diseases. In this study, we examined the neuroprotective effect of SP600125, a selective JNK inhibitor, in cerebellar granule cells (CGNs) deprived of serum and
A Jóźwik et al.
Veterinary research communications, 29(4), 347-359 (2005-03-09)
Two pairs of primers were prepared, both localized within the sequences of the nucleoprotein gene (NP) of canine distemper virus (CDV). A number of experiments were done to optimize the conditions of RT-PCR and nested PCR methods. The nucleic acids
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Protocolli

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Amplification of Genomic DNA using REDTaq® DNA Polymerase

Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.

Contenuto correlato

Numero articolo commerciale globale

SKUGTIN
D6063-250UN04061833587249

Domande

Recensioni

Nessuna valutazione

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