RAJI Cell Line human

85011429, human lymph, Lymphoblastoid

• Sinonimo/i: GM04671 Cells, P1-Raji Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: RAJI Cell Line human, 85011429
• Origine biologica: human lymph
• Descrizione: Human Black, Burkitt′s lymphoma
• Modalità di accrescimento: Suspension
• Cariotipo: 2n = 46, diploid
• Morfologia: Lymphoblastoid
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Burkitt′s lymphoma

Descrizione della linea cellulare

Established in 1963 from Burkitt′s lymphoma in an 11 year old male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a ′non-producer′; the EBV genome carries deletions attributed to preventing the formation of virus particles. Ethnicity: Black.

Applicazioni

Resistance and virus studies: vesicular stomatitis virus (VSV).
RAJI Cell Line human has been used to investigate the transport of V. parahaemolyticus across the Payer′s patch of the intestinal epithelium and it has been co-cultured with Caco-2 (human colorectal adenocarcinoma cell-line) monolayers to study bacterial translocation.

Profilo DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 16,19

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Altre note

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