C9179
Monoclonal Anti-Cytokeratin Peptide 17 antibody produced in mouse
clone CK-E3, ascites fluid
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About This Item
Prodotti consigliati
Origine biologica
mouse
Coniugato
unconjugated
Forma dell’anticorpo
ascites fluid
Tipo di anticorpo
primary antibodies
Clone
CK-E3, monoclonal
contiene
15 mM sodium azide
Reattività contro le specie
rat, human, bovine, pig, goat
tecniche
indirect immunofluorescence: 1:400 using acetone-fixed frozen sections of human salivary gland
microarray: suitable
western blot: suitable
Isotipo
IgG2b
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... KRT17(3872)
Descrizione generale
Cytokeratin 17, with a molecular weight of 46 kDa, is a member of the type I acidic subfamily of cytokeratins that is expressed in normal human epithelial tissues such as glandular epithelium with myoepithelial component, transitional and pseudostratified epithelia. This protein may be involved in the generation of spatial organization of epithelial tissues.
Specificità
Recognizes specifically human cytokeratin 17 (46-47 kDa) and rat cytokeratin 19 (40 kDa). The antibody labels intermediate filaments in human cultured epitheloid carcinoma cell line (HeLa) and in rat hepatoma cells, but does not stain rat fibroblasts in primary cultures. It stains a minor basal cell population in all variants of complex epithelia; strong staining was noted in myoepithelial cells of all glands studied. The antibody exhibits a wide interspecies cross-reactivity, however, the recognized cytokeratin was not studied in cases other than human and rat. The epitope detected by the antibody is sensitive to formalin fixation.
Immunogeno
cytoskeletal preparation from rat colon epithelium.
Applicazioni
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Monoclonal Anti-Cytokeratin Peptide 17 antibody produced in mouse is suitable for western blotting, microarray and indirect immunofluorescence at a working dilution of 1:400 using acetone-fixed frozen sections of human salivary gland. It recognizes an epitope present in epithelial tissues and may be used for typing of normal, metaplastic and neoplastic cells. It may be useful in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It may also be used for the localization of cytokeratin 17 using various immunochemical assays such as dot blotting and immunohistochemistry. It has been used in the detection of expression of inducible keratin k17 in human keratinocytes in pachyonychia congenita patients.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Paul E Bowden et al.
Veterinary dermatology, 20(5-6), 347-359 (2010-02-25)
Canine claws are complex epithelial structures resembling the mammalian hair fibre, and human nail plate, in terms of tissue-specific differentiation. They are composed of several distinct epithelial cell lineages undergoing either hard or soft keratinization. The claw plate has three
John A Wisler et al.
Toxicologic pathology, 39(5), 809-822 (2011-06-17)
Seven novel and potent Raf small molecule kinase inhibitors (C1-7) were evaluated in seven-day oral repeat dose rat toxicity studies. All compounds tested induced hyperplasia in multiple tissues. Consistently affected was stratified squamous epithelium at a number of sites and
In vitro differentiation of human tooth germ stem cells into endothelial- and epithelial-like cells.
Ayşegül Doğan et al.
Cell biology international, 39(1), 94-103 (2014-08-01)
Current clinical techniques in dental practice include stem cell and tissue engineering applications. Dental stem cells are promising primary cell source for mainly tooth tissue engineering. Interaction of mesenchymal stem cell with epithelial and endothelial cells is strictly required for
Robyn P Hickerson et al.
Journal of dermatological science, 56(2), 82-88 (2009-08-25)
The macrolide sirolimus (rapamycin) selectively blocks translation of mRNAs containing a terminal 5' oligopyrimidine (TOP) tract by altering the activity of mammalian target of rapamycin (mTOR) and inhibiting downstream mTOR pathway components involved in TOP mRNA translation. The skin disorder
S M Troyanovsky et al.
Journal of cell science, 93 ( Pt 3), 419-426 (1989-07-01)
By immunomorphology, using keratin 17-specific monoclonal antibody, it has been shown that this keratin is expressed only in the basal cells of a group of complex epithelia: glandular epithelium with myoepithelial component, transitional and pseudostratified epithelia. Immunolocalization of keratin 17
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