N8664

Anti-NONO (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-NMT55, Anti-NRB54, Anti-Non-pou domain-containing octamer-binding protein, Anti-Nuclear RNA-binding protein, 54-KD, Anti-p54nrb
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• PM: antigen ~55 kDa
• Reattività contro le specie: human
• Concentrazione: ~1.0 mg/mL
• tecniche: immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates; indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HeLa cells; western blot: 1-2 μg/mL using NIH-3T3 cell lysates
• N° accesso UniProt: Q15233
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... NONO(4841)

Descrizione

Descrizione generale

Non-POU domain containing octamer binding (NONO) is ubiquitously expressed and comprises of two tandem RNP-type RNA- recognition motifs and a putative helix-turn-helix domain followed by a highly charged region. It is enriched in paraspeckles, a nucleoplasmic compartment and relocalizes to cap structures at the nucleolar periphery when transcription is inhibited.

Non-POU domain containing octamer binding protein (NONO) is part of the Drosophila behavior/human splicing (DBHS) protein family. It has a molecular weight of 54kDa and the gene encoding it is localized on human chromosome Xq13.1.

Applicazioni

Anti-NONO (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Anti-NONO (N-terminal) antibody produced in rabbit has been used in immunoprecipitation.

Azioni biochim/fisiol

NONO (also termed as p54nrb) is a nuclear protein implicated in numerous processes including transcription, pre-mRNA processing, nuclear retention of edited RNA and DNA relaxation. NONO was suspected to be involved in pre-mRNA splicing. Later it was found that NONO and PSF mediate different functions depending on their intracellular location.

Non-POU domain containing octamer binding protein (NONO) modulates transcription. Loss-of-function of the protein has been linked to human intellectual disability. The protein also has a role in synaptic transcription and regulation of the circadian clock. NONO also has a function in DNA damage response.

Stato fisico

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable