C-12225

Human Dermal Blood Endothelial Cells (HDBEC)

Adult, 500,000 cryopreserved cells

• Codice UNSPSC: 41106514
• NACRES: NA.81

Proprietà

• Origine biologica: human dermis
• Confezionamento: pkg of 500,000 cells
• Morfologia: ( endothelial)
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cardiovascular diseases; cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Descrizione generale

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Origine della linea cellulare

Skin

Applicazioni

Primary Human Dermal Blood Endothelial Cells (HDBEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of adult skin (different locations) from a single donor and are provided in a cryopreserved format. (Human Dermal Lymphatic Endothelial Cells (HDLEC) from the same donor are available on request.)The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: they are >95% CD31 positive and stain positive for von Willebrand factor. In addition, they are >90% negative for podoplanin and stain negative for smooth muscle specific alpha-actin.Blood Endothelial Cells have a key function in physiological processes like vessel tonus, capillary permeability, blood coagulation, fibrolysis, and angiogenesis. They are also useful for disease studies of atherosclerosis, tumor genesis, and thrombosis.

Qualità

Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested forthe absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Attenzione

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Mantenimento delle subcolture

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Altre note

Recommended Plating Density: 10000 - 20000 cells per cm²Passage After Thawing: P2Tested Markers: Podoplanin negative, CD31 positiveGuaranteed population doublings: >15

Prodotti consigliati

Recommended Primary Cell Culture Media:Link

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable