Potassium buffer is not required. For assay purposes, this product is reconstituted in potassium phosphate buffer, pH 6.0, at 0.2 mg/ml, Solutions may also be prepared 40 mM Tris HCL, pH 7.5, containing 50 mM NaCl and 50% glycerol. This stock may be stored for extended periods at –20 °C. Other sources indicate that this enzyme may also be prepared in water containing 0.01% BSA and TBE buffer. These options have not been validated. Please see the link below to review a publication that may be of interest:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817930/
A6306
Agarase from Pseudomonas atlantica
lyophilized powder, ≥5,000 units/mg protein (Lowry)
Sinonimo/i:
β-Agarase, Agarose 4-glycanohydrolase
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1000 UNITS
367,00 €
5000 UNITS
814,00 €
367,00 €
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1000 UNITS
367,00 €
5000 UNITS
814,00 €
About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
eCl@ss:
42040102
NACRES:
NA.26
367,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Origine biologica
bacterial (Pseudomonas atlantica)
Livello qualitativo
Stato
lyophilized powder
Attività specifica
≥5,000 units/mg protein (Lowry)
Temperatura di conservazione
2-8°C
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Categorie correlate
Definizione di unità
One unit will produce 1.0 μg of reducing sugar (measured as D-galactose) from agar per min at pH 6.0 at 40 °C.
Stato fisico
Product is formulated with phosphate buffered salts, bovine serum albumin and Agarase. Total protein content ranges between 10 – 30% w/w.
Altre note
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Will this enzyme work in water or PBS, or must it be a potassium buffer (for which not further details are provided in the spec sheet)?
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