5162
Bovine Collagen Type I
from bovine flexor tendon, powder, suitable for cell culture, used for 3D gel formation
Sinonimo/i:
Collagen Protein, Collagen Solution, Collagen Type I
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1 G
208,00 €
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Cambia visualizzazione
1 G
208,00 €
About This Item
Prodotti consigliati
Nome del prodotto
Collagen, from bovine flexor tendon
Origine biologica
bovine flexor tendon
Sterilità
non-sterile
Saggio
>96%
Stato
lyophilized powder (fibrous)
Confezionamento
pkg of 1 g
tecniche
cell culture | mammalian: suitable
N° accesso UniProt
Specificità del legame
Peptide Source: Elastin
Peptide Source: Fibronectin
Peptide Source: Laminin
Condizioni di spedizione
wet ice
Temperatura di conservazione
room temp
Informazioni sul gene
bovine ... COL2A1(407142)
Descrizione generale
This product is supplied as a lyophilized fibrous powder in a 1 gram package size. Bioburden and endotoxin levels are tested – this product is not considered sterile.
This collagen product is naturally cross-linked yielding a robust material for applications which require structure and strength. This product can be readily prepared into such forms as tissue scaffolds, foams, sponges, suspensions, coatings, putties, films and sheets but does not form hydrogels. Using typical cross-linking methods, this material can be tuned for optimal in vivo resorption. This collagen product is ideal for tissue engineering applications and uses with inorganic and biomaterials.
The product is provided in user-friendly packaging for use and storage. Avoid extended exposure to ambient environment since this material is hygroscopic.
This collagen product is naturally cross-linked yielding a robust material for applications which require structure and strength. This product can be readily prepared into such forms as tissue scaffolds, foams, sponges, suspensions, coatings, putties, films and sheets but does not form hydrogels. Using typical cross-linking methods, this material can be tuned for optimal in vivo resorption. This collagen product is ideal for tissue engineering applications and uses with inorganic and biomaterials.
The product is provided in user-friendly packaging for use and storage. Avoid extended exposure to ambient environment since this material is hygroscopic.
Nota sulla preparazione
Type I bovine collagen, insoluble lyophilized fibrous powder, is extracted from bovine flexor tendon with the raw material sourced from closed/controlled herds of animals. This product is very difficult to dissolve, and after following the below protocol you will end up with a viscous solution.
Note: The following procedure is based on solubilization of 1 gram of collagen in 100 ml to initially prepare a 10 mg/ml collagen suspension. Smaller quantities and volumes may be used but the same ratios of collagen and solutions should be used.
1. Weigh out 1 gram of collagen fibrous powder.
2. Reconstitute 1 gram of collagen with 50 ml of cold purified water (50% of the final volume).
3. Stir the collagen with the water continuously mixing for a minimum of 15 minutes until the collagen is fully wetted and the suspension appears to be a semi-solution.
4. Add 50 ml of cold 0.02 M HCl to the collagen mixture and stir for a minimum of 10 minutes. This will yield a collagen concentration of 10 mg/ml with the suspension continuing to appear as a semi-solution.
5. Measure the pH - the mixture should have a pH of 2 to 3.
6. Using Waring stick blender or equivalent, homogenize the collagen mixture for a minimum of 15 minute at a high speed ensuring that the collagen is fully homogenized. Ensure that the temperature of mixture does exceed 24°C. Upon completion, there should be no visual solids in the viscous suspension. Air bubbles with be prevalent.
7. To remove air bubbles, stir the solution on a stir plate and pull a vacuum on the suspension. This will remove the air bubbles.
8. At this point if a collagen concentration of less than 10 mg/ml is desired, the collagen can be diluted with 0.01 M HCl.
Note: The following procedure is based on solubilization of 1 gram of collagen in 100 ml to initially prepare a 10 mg/ml collagen suspension. Smaller quantities and volumes may be used but the same ratios of collagen and solutions should be used.
1. Weigh out 1 gram of collagen fibrous powder.
2. Reconstitute 1 gram of collagen with 50 ml of cold purified water (50% of the final volume).
3. Stir the collagen with the water continuously mixing for a minimum of 15 minutes until the collagen is fully wetted and the suspension appears to be a semi-solution.
4. Add 50 ml of cold 0.02 M HCl to the collagen mixture and stir for a minimum of 10 minutes. This will yield a collagen concentration of 10 mg/ml with the suspension continuing to appear as a semi-solution.
5. Measure the pH - the mixture should have a pH of 2 to 3.
6. Using Waring stick blender or equivalent, homogenize the collagen mixture for a minimum of 15 minute at a high speed ensuring that the collagen is fully homogenized. Ensure that the temperature of mixture does exceed 24°C. Upon completion, there should be no visual solids in the viscous suspension. Air bubbles with be prevalent.
7. To remove air bubbles, stir the solution on a stir plate and pull a vacuum on the suspension. This will remove the air bubbles.
8. At this point if a collagen concentration of less than 10 mg/ml is desired, the collagen can be diluted with 0.01 M HCl.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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