Atto 550 Protein Labeling Kit

Name: Atto 550 Protein Labeling Kit
Brand: SIGMA

BioReagent, suitable for fluorescence

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NACRES:

NA.32

UNSPSC Code:

12352200

Storage temp.:

2-8°C

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product line

BioReagent

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λ_ex 544 nm; λ_em 576 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)

suitability

suitable for fluorescence

storage temp.

2-8°C

Quality Level

100

Categorie correlate

Detection Substrates & Enzymes

Proteins & Enzymes

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Questo articolo	68616	76508	38371
Sigma-Aldrich 51146 Atto 550 Protein Labeling Kit	Sigma-Aldrich 68616 Atto 594 Protein Labeling Kit	Sigma-Aldrich 76508 Atto 647N Protein Labeling Kit	Sigma-Aldrich 38371 Atto 488 Protein Labeling Kit
fluorescence λ_ex 544 nm; λ_em 576 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)	fluorescence λ_ex 594 nm; λ_em 625 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)	fluorescence λ_ex 647 nm; λ_em 661 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)	fluorescence λ_ex 488 nm; λ_em 520 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)
manufacturer/tradename ATTO-TEC GmbH	manufacturer/tradename ATTO-TEC GmbH	manufacturer/tradename ATTO-TEC GmbH	manufacturer/tradename ATTO-TEC GmbH
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
product line BioReagent	product line BioReagent	product line BioReagent	product line BioReagent
suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence

General description

This kit contains sufficient amounts of reactive dye, buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies. Atto 550 Protein Labeling Kit contains Atto 550 succinimidyl ester which enables conjugation of Atto 550 to the primary amino groups of the proteins and other molecules.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Classe di stoccaggio

10 - Combustible liquids

wgk

WGK 3

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