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Sigma-Aldrich

Atto 550

for fluorescence, ≥90% (HPLC)

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About This Item

Numero MDL:
MFCD05865402
Codice UNSPSC:
12352108
NACRES:
NA.32

Grado

for fluorescence

Saggio

≥90% (HPLC)

Forma fisica

powder

Produttore/marchio commerciale

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

Assorbanza UV

λ: 553-559 nm Amax

Temperatura di conservazione

−20°C

Descrizione generale

Atto 550 is a new label with high molecular absorption (120,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. Atto 550 can be used with similar excitation source and fluorescence filters as Cy3® and is characterized by a high photostability.

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Applicazioni

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3™, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies.

Note legali

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
CAB-O-SIL is a registered trademark of Cabot Corp.
Cy3 is a trademark of Cytiva

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
Antibody transfection into neurons as a tool to study disease pathogenesis.
Douglas JN, Gardner LA, et al.
Journal of Visualized Experiments, 26, 4154-4154 (2012)
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Nature methods, 5(6), 507-516 (2008-05-31)
Single-molecule fluorescence resonance energy transfer (smFRET) is one of the most general and adaptable single-molecule techniques. Despite the explosive growth in the application of smFRET to answer biological questions in the last decade, the technique has been practiced mostly by
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We present a test case example of a detailed single-molecule fluorescence study of one of the most sophisticated and complex DNA devices introduced to date, a recently published autonomous bipedal DNA motor. We used the diffusion-based single-molecule Förster resonance energy
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