The protocol for this detection to run this antibody in that manner. The protocol available is with UltraView detection and No Pretreatment, a 32-minute antibody incubation at room temperature, plus amplifier.
202M-9
Actin, Smooth Muscle (1A4) Mouse Monoclonal Antibody
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About This Item
Codice UNSPSC:
12352203
NACRES:
NA.41
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
100
500
Coniugato
unconjugated
Forma dell’anticorpo
culture supernatant
Tipo di anticorpo
primary antibodies
Clone
1A4, monoclonal
Descrizione
For In Vitro Diagnostic Use in Select Regions (See Chart)
Stato
buffered aqueous solution
Reattività contro le specie
human
Confezionamento
vial of 0.1 mL concentrate (202M-94)
vial of 0.5 mL concentrate (202M-95)
bottle of 1.0 mL predilute (202M-97)
vial of 1.0 mL concentrate (202M-96)
bottle of 7.0 mL predilute (202M-98)
Produttore/marchio commerciale
Cell Marque®
tecniche
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
Isotipo
IgGκ
Controllo
appendix
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Visualizzazione
cytoplasmic
Categorie correlate
Descrizione generale
Smooth Muscle Actin is a part of the actin family of proteins which are highly conserved andform microfilaments. These filaments are one of the major components of the cytoskeleton.Anti-smooth muscle actin immunohistochemical reactivity is seen in smooth muscle cells,myofibroblasts, and myoepithelial cells.
Qualità
 IVD |  IVD |  IVD |  RUO |
Linkage
Actin, Smooth Muscle Positive Control Slides, Product No. 202S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).
Stato fisico
Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
Nota sulla preparazione
Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.
Altre note
For Technical Service please contact: 800-665-7284 or email: [email protected]
Note legali
Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany
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Maria Delia Perez-Montiel et al.
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The diagnosis of low-grade and pseudosarcomatous spindle cell lesions of skin and soft tissue can sometimes be problematic; in particular, distinction between fibroblastic, myofibroblastic, and smooth muscle proliferations can occasionally pose difficulties on routine histologic examination. We have applied a
M Kuroda
Biochimica et biophysica acta, 843(3), 208-213 (1985-12-13)
Changes of actin isomers during development and differentiation of chicken gizzard were investigated by polyacrylamide gel electrophoresis. The two-dimensional gel electrophoresis with SDS-polyacrylamide gels in the presence of urea as the second dimension clearly separated three actin isomers which appear
Immunofluorescence studies on the structure of actin filaments in tissue culture cells.
E Lazarides
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 23(7), 507-528 (1975-07-01)
P Cooke
The Journal of cell biology, 68(3), 539-556 (1976-03-01)
There are three classes of myofilaments in vertebrate smooth muscle fibers. The thin filaments correspond to actin and the thick filaments are identified with myosin. The third class of myofilaments (100 A diam) is distinguished from both the actin and
O Skalli et al.
The Journal of cell biology, 103(6 Pt 2), 2787-2796 (1986-12-01)
A monoclonal antibody (anti-alpha sm-1) recognizing exclusively alpha-smooth muscle actin was selected and characterized after immunization of BALB/c mice with the NH2-terminal synthetic decapeptide of alpha-smooth muscle actin coupled to keyhole limpet hemocyanin. Anti-alpha sm-1 helped in distinguishing smooth muscle
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Can Smooth Muscle Actin (1A4) be run on the Ventana/Roche Ultra using OptiView detection, and what would be the protocol for this?
1 risposta-
Utile?
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