Passa al contenuto
Merck
Tutte le immagini(1)

Documenti fondamentali

11004760001

Roche

Random Primed DNA Labeling Kit

Sinonimo/i:

nucleic acid labeling

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali

Scegli un formato

50 REACTIONS
578,00 €

578,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.


Scegli un formato

Cambia visualizzazione
50 REACTIONS
578,00 €

About This Item

Codice UNSPSC:
41105500

578,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

impiego

sufficient for 50 labeling reactions

Livello qualitativo

Produttore/marchio commerciale

Roche

Temperatura di conservazione

−20°C

Categorie correlate

Descrizione generale

Random primed DNA labeling kit is used for the radioactive and nonradioactive labeling of DNA with modified deoxyribonucleoside triphosphate using random oligonucleotides as primers. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers.

Specificità

Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or by heating to 65 °C for 10 minutes.

Applicazioni

Random Primed DNA Labeling Kit has been used to label probe or fragments shorter than 1 kb.[1]
Random Primed DNA Labeling Kit is used to uniformly label plasmid or phage DNA with any [α-32P]-dNTP or modified dNTP. Labeled DNA probes with high specific activity are used in a variety of hybridization techniques including:
  • Screening of gene libraries
  • Southern, dot and northern blots[2][3]
  • In situ hybridizations

Confezionamento

1 kit containing 7 components.

Nota sulla preparazione

Working solution: Preparation dNTP Stock Mix
To avoid pipetting inaccuracy, due to low volumes, prepare a stock mix of unlabeled dNTPs. Aliquots should be stored at -15 to -25 °C. Avoid repeated freezing and thawing.
  • dATP, dGTP, dTTP mixture:
    For one labeling reaction pipette:
    1 μl dATP
    1 μl dGTP
    1 μl dTTP to a reaction vial

Preparation of DIG Stock Mix
To avoid pipetting inaccuracy due to low volumes, prepare a DIG stock mix. To do this, mix digoxigenin-11-dUTP [3 mM] and dTTP (vial 5) 1:1. For each labeling reaction 1.6 μl are used.

Altre note

For life science research only. Not for use in diagnostic procedures.

Solo come componenti del kit

N° Catalogo
Descrizione

  • Control DNA lambda, 20 μl 12.5 µg/ml

  • dATP, 50 μl 0.5 mM

  • dCTP, 50 μl 0.5 mM

  • dGTP, 50 μl 0.5 mM

  • dTTP, 50 μl 0.5 mM

  • Hexanucleotide mixture in 10x reaction buffer (100 μl)

  • Klenow enzyme, labeling grade (100 U)

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash


Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number

Non trovi la versione di tuo interesse?

Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 1

1 of 1

I J Blader et al.
The Journal of biological chemistry, 276(26), 24223-24231 (2001-04-11)
Cells infected with the intracellular protozoan parasite Toxoplasma gondii undergo up-regulation of pro-inflammatory cytokines, organelle redistribution, and protection from apoptosis. To examine the molecular basis of these and other changes, gene expression profiles of human foreskin fibroblasts infected with Toxoplasma
Daniel R Semlow et al.
Cell, 167(2), 498-511 (2016-10-04)
During eukaryotic DNA interstrand cross-link (ICL) repair, cross-links are resolved ("unhooked") by nucleolytic incisions surrounding the lesion. In vertebrates, ICL repair is triggered when replication forks collide with the lesion, leading to FANCI-FANCD2-dependent unhooking and formation of a double-strand break
Fluorescence in situ hybridization to the polytene chromosomes of anopheles mosquitoes
He F, et al.
PLoS Pathogens (2012)
Masayuki Kimura et al.
Nature protocols, 5(9), 1596-1607 (2010-11-19)
In this protocol we describe a method to obtain telomere length parameters using Southern blots of terminal restriction fragments (TRFs). We use this approach primarily for epidemiological studies that examine leukocyte telomere length. However, the method can be adapted for
Margit Dlaska et al.
Cell cycle (Georgetown, Tex.), 12(13), 2084-2099 (2013-06-14)
Immortal cells require a mechanism of telomere length control in order to divide infinitely. One mechanism is telomerase, an enzyme that compensates the loss of telomeric DNA. The second mechanism is the alternative lengthening of telomeres (ALT) pathway. In ALT

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.