10874515001

Bestatin

95% (HPLC), crystalline powder, suitable for protein purification

• Sinonimo/i: Bestatin hydrochloride, N-[(2S,3R)-3-Amino-2-hydroxy-4-phenylbutyryl]-L-leucine hydrochloride, N-[(2S,3R)-3-Amino-2-hydroxy-4-phenylbutyryl]-L-leucine hydrochloride
• Formula empirica (notazione di Hill): C₁₆H₂₄N₂O₄ · HCl
• Numero CAS: 65391-42-6
• Peso molecolare: 344.83
• Beilstein: 4628066
• Numero MDL: MFCD00058004
• Codice UNSPSC: 12352204
• ID PubChem: 329749214

Proprietà

• Descrizione: [(2S,3R)-3-Amino-2-hydroxy-4-phenylbutanoyl]-L-leucine hydrochloride
• Saggio: 95% (HPLC)
• Forma fisica: crystalline powder
• Confezionamento: pkg of 10 mg
• Produttore/marchio commerciale: Roche
• Condizioni di stoccaggio: protect from light
• tecniche: protein purification: suitable
• Punto di fusione: 195-205 °C
• Solubilità: water: soluble 25 g/L; alcohol: soluble 5 g/L
• λmax: 255-260 nm at 1 N in hydrochloric acid
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: O=C(N[C@@H](CC(C)C)C(O)=O)[C@@H](O)[C@H](N)CC1=CC=CC=C1.Cl[H]
• InChI: 1S/C16H24N2O4.ClH/c1-10(2)8-13(16(21)22)18-15(20)14(19)12(17)9-11-6-4-3-5-7-11;/h3-7,10,12-14,19H,8-9,17H2,1-2H3,(H,18,20)(H,21,22);1H/t12-,13+,14+;/m1./s1; XGDFITZJGKUSDK-UDYGKFQRSA-N

Descrizione

Applicazioni

Bestatin has been used as a protease inhibitor for the purification of his-tagged Tau protein.

Azioni biochim/fisiol

A metalloprotease inhibitor selective for aminopeptidase. Bestatin is a competitive and specific inhibitor of leucine aminopeptidase, aminopeptidase B, and triamino peptidase. It inhibits aminopeptidase B at 60 nM (using arginine-β-naphthylamide as substrate) and leucine aminopeptidase at 20 nM (leucine-β-naphthylamide as substrate). It showed no inhibition of aminopeptidase A, trypsin, chymotrypsin, elastase, papain, pepsin, or themolysin. It offers promise as a novel analgesic because it protects endogenous opioid peptides against degradation.

Bestatin exhibits anti-angiogenic property and inhibits new blood vessel formation at the early stage of tumor growth and in the metastatic sites.

Nota sulla preparazione

Working concentration: 40 μg/ml (130 μM)
Working solution: Recommended solvents are 1 M HCl, methanol or 0.15 M NaCl.
Soluble to 20 mg/ml in 1 M HCl, 5 mg/ml in methanol, and 1 mg/ml in 0.15 M NaCl. Do not store in HCl.
For best results, use a stock solution of 2–5 mg/ml in methanol.
Storage conditions (working solution): -15 to -25 °C
Avoid repeated freezing!

Ricostituzione

Soluble to 20 mg/ml in 1 M HCl, 5 mg/ml in methanol, and 1 mg/ml in 0.15 M NaCl. Do not store in HCl. Roche recommends preparing a stock solution of 2 to 5 mg/ml in methanol.
Solutions are stable for 6 months when stored in aliquots at -15 to -25 °C.

Stoccaggio e stabilità

Store at 2 to 8 °C. (Store dry!)

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable