Yes, this product is suitable for use with suspension cells.
SCT210
BioTracker FerroOrange Live Cell Dye
Live Cell imaging probe that specifically detects labile ferrous (Fe2+) ions in live cells.
Sinonimo/i:
senescent cells IHC kit
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165,00 €
Spedizione prevista il17 aprile 2025Dettagli
Scegli un formato
About This Item
165,00 €
Spedizione prevista il17 aprile 2025Dettagli
Prodotti consigliati
Stato
lyophilized
applicazioni
cell analysis
Metodo di rivelazione
fluorometric
Categorie correlate
Descrizione generale
Esclusione di responsabilità
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Can this kit be used for suspension cells?
1 risposta-
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Is it possible to store SCT210 after reconstitution?
1 risposta-
The available datasheet for SCT210 provides guidance in the Reagent Preparation section, stating, "Use neutral buffer or culture medium for dilution of the DMSO solution and use immediately upon dilution. The dye could be oxidized in acidic solutions." However, there are no recommendations or data suggesting that the solutions are stable when stored. It's important to note that the package is shipped in single-use vials.
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Is it possible to detect SCT210 FerroOrange by flow cytometry?
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The website indicates an excitation wavelength of 542 nm and an emission wavelength of 572 nm for the SCT210 FerroOrange. The product is claimed to be suitable for live cell imaging, which typically implies the use of a confocal microscope. However, given that this is a fluorescent dye, it seems reasonable to consider that the staining could be assessed using a flow cytometer. There is currently no available data documenting its use on a flow cytometer. To use it on a flow cytometer, the cells would need to be in a single cell suspension. Additionally, it is expected that the cells could be visualized using a fluorescent microscope.
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What cells can be used as a positive control for SCT210 (BioTracker™ FerroOrange Live Cell Dye)?
1 risposta-
The HepG2 cell line is recommended as a positive control for SCT210 (BioTracker™ FerroOrange Live Cell Dye).
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