OP03L

Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)

lyophilized, clone PAb421, Calbiochem^®

• Codice UNSPSC: 12352203
• NACRES: NA.43

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: PAb421, monoclonal
• Stato: lyophilized
• non contiene: preservative
• Reattività contro le specie: human, monkey, mouse, rabbit, rat
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Isotipo: IgG2a
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TP53(7157)

Descrizione

Descrizione generale

Purified mouse monoclonal antibody, originally called clone L21, generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type and mutant forms of p53.

Recognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated p53.

This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).

Immunogeno

Epitope: within amino acids 376-378 of human p53

Mouse

partially purified mouse p53

Applicazioni

Frozen Sections (10 µg/ml, see application references)

Gel Shift (see comments)

Immunoblotting (10 µg/ml, see application references)

Immunofluorescence (1-20 µg/ml, see application references)

Flow Cytometry (1-20 µg/ml)

Immunoprecipitation (1 µg per sample, see application references)

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

Lyophilized from a volatile buffer, 100 µg BSA.

Ricostituzione

Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide; freezing of aliquots is best for long-term storage of reconstituted product, but repetitive freezing and thawing should be avoided.

Risultati analitici

Negative Control
Normal skin tissue or SK-OV-3 cells

Positive Control
Breast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)

Altre note

El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Legros, Y., et al. 1994. Oncogene9, 2071.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Kastan, M.B., et al. 1992. Cell71, 587.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51 6304.
Crawford, L. and Harlow, E. 1982. J. Virol.41, 709.
Harlow, E., et al. 1981. J. Virol.39 861.

Originally called clone L21. Does not recognize phosphorylated or acetylated p53. For a gel shift assay, resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with ³⁵S-Met (≤1 h) will help reduce background. For immunoblotting wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then perform immunoblotting using Cat. No. PC35 and chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1