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NE1004

Sigma-Aldrich

Anti-Apolipoprotein E Mouse mAb (E6D7)

liquid, clone E6D7, Calbiochem®

Sinonimo/i:

Anti-ApoE

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100 μL
670,00 €

670,00 €


Spedizione prevista il28 aprile 2025Dettagli


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Cambia visualizzazione
100 μL
670,00 €

About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

670,00 €


Spedizione prevista il28 aprile 2025Dettagli


Richiedi un ordine bulk

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

culture supernatant

Tipo di anticorpo

primary antibodies

Clone

E6D7, monoclonal

Stato

liquid

non contiene

preservative

Reattività contro le specie

human

Non deve reagire con

mouse, rabbit

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze
avoid repeated freeze/thaw cycles

Isotipo

IgG1

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... APOE(348)

Descrizione generale

Anti-Apolipoprotein E, mouse monoclonal, clone E6D7, recognizes the ~35 kDa Apo E2, E3, and E4 in human serum and brain. It is validated for ELISA, WB, IP, and paraffin and frozen sections.
Mouse monoclonal antibody supplied as diluted culture supernatant. Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E.
Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum and brain.

Immunogeno

Human
a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE

Applicazioni




ELISA (see comments)
Immunoblotting (1:100-1:1000)
Immunoprecipitation (see comments)
Frozen Sections (1:100-1:10,000)
Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

Culture supernatant diluted in PBS.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C).

Risultati analitici

Positive Control
Brain tissue, serum

Altre note

Golabek, A.A., et al. 1996. J. Biol. Chem.271, 10602.
Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(3), 685-700 (2016-01-23)
A major unanswered question in biology and medicine is the mechanism by which the product of the apolipoprotein E ε4 allele, the lipid-binding protein apolipoprotein E4 (ApoE4), plays a pivotal role in processes as disparate as Alzheimer's disease (AD; in
Dale J Christensen et al.
Journal of immunology (Baltimore, Md. : 1950), 186(4), 2535-2542 (2011-02-04)
The molecular mechanism by which apolipoprotein E (apoE) suppresses inflammatory cytokine and NO production is unknown. Using an affinity purification approach, we found that peptide mimetics of apoE, derived from its receptor binding domain residues 130-150, bound to the SET
Xia Hu et al.
Frontiers in neuroanatomy, 11, 45-45 (2017-06-24)
Genetic variations in the vacuolar protein sorting 10 protein (Vps10p) family have been linked to Alzheimer's disease (AD). Here we demonstrate deposition of fragments from the Vps10p member sortilin at senile plaques (SPs) in aged and AD human cerebrum. Sortilin
Jing Zhang et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(6), 7748-7757 (2019-03-22)
Aging and apolipoprotein E4 (ApoE4) can increase the risk of cognitive impairment and neurodegenerative disorders, including Alzheimer's disease (AD), and patients with type 2 diabetes mellitus are highly susceptible to cognitive dysfunction. Recent research has indicated that metformin, a prescribed

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