NC010
Nerve Growth Factor 7.0s
Sinonimo/i:
holo-NGF
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About This Item
Codice UNSPSC:
12352207
eCl@ss:
32160405
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Saggio
97% (SDS-PAGE)
Stato
solid
Produttore/marchio commerciale
Chemicon®
Concentrazione
1 mg/mL
N° accesso UniProt
Condizioni di spedizione
dry ice
Informazioni sul gene
mouse ... KLK4(56640)
Descrizione generale
In the mouse submaxillary gland the neurotrophically active NGF, beta-NGF (2.5S), is part of a larger protein complex, 7S-NGF, which has the stoichiometry alpha2betagamma2 (+ 1 or 2 zinc ions) where the alpha and gamma subunits are members of the glandular kallikrein family of serine proteases, each of about 26 000 kDa molecular weight. The alpha and gamma subunits actually inhibit beta-NGF action and must be dissociated for biological activity to be observed.
This 7S complex has not been shown to occur in other mouse tissues, or in other species, probably because the alpha and gamma subunits, which are members of a larger glandular kallikrein family, apparently have a very restricted expression. The alpha and gamma subunits, members of the serine proteinase family, have quite similar amino acid sequences, although only the gamma subunit is catalytically active. The alpha subunit has undergone several mutations that preclude this function, including loss of the cleavage site needed for processing from the zymogen to the active form.
This 7S complex has not been shown to occur in other mouse tissues, or in other species, probably because the alpha and gamma subunits, which are members of a larger glandular kallikrein family, apparently have a very restricted expression. The alpha and gamma subunits, members of the serine proteinase family, have quite similar amino acid sequences, although only the gamma subunit is catalytically active. The alpha subunit has undergone several mutations that preclude this function, including loss of the cleavage site needed for processing from the zymogen to the active form.
MOLECULAR WT.:
130-140 kD. 7S NGF is composed of 3 subunits; 1 beta subunit (2.5S), 2 alpha subunits and 2 gamma subunits.
130-140 kD. 7S NGF is composed of 3 subunits; 1 beta subunit (2.5S), 2 alpha subunits and 2 gamma subunits.
Product Source: Mouse submaxillary gland (Darling & Shooter, 1984).
Applicazioni
The recommended concentration to be used in vitro for maintenance of sympathetic and immature sensory nerve cells is 50-100 ng/mL medium (Varon et al., 1967).
Stato fisico
Lyophilized. NC010 should be reconstituted with sterile DMEM at a concentration of
10 μg/mL and stored at -70°C. Medium with mNGF cannot be reused due to mNGF degradation.
10 μg/mL and stored at -70°C. Medium with mNGF cannot be reused due to mNGF degradation.
Stoccaggio e stabilità
Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Risultati analitici
Specific Activity: The neurotrophic activity is in the range of 0.5-50 ng/mL using rat PC12 cells cultured for 7-14 days
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
The isolation of the mouse nerve growth factor protein in a high molecular weight form.
S Varon et al.
Biochemistry, 6(7), 2202-2209 (1967-07-01)
Filtri attivi
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