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MABT186

Sigma-Aldrich

Anti-Exo70 Antibody, clone 70X13F3

clone 70X13F3, from mouse

Sinonimo/i:

Exocyst complex component 7, Exocyst complex component Exo70, rExo70

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100 μL
435,00 €

435,00 €


Spedizione prevista il09 maggio 2025Dettagli


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Cambia visualizzazione
100 μL
435,00 €

About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

435,00 €


Spedizione prevista il09 maggio 2025Dettagli


Richiedi un ordine bulk

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

70X13F3, monoclonal

Reattività contro le specie

human, rat

tecniche

immunocytochemistry: suitable
western blot: suitable

Isotipo

IgG2bκ

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... EXOC7(23265)

Descrizione generale

The exocyst complex component 70 (Exo70) is a component of the exocyst complex, an octameric structure that is also comprised of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84. The exocyst complex facilitates the fusion of secretory vesicles with the plasma membrane and the release of vesicular cargo, which may include neurotransmitters, hormones, and membrane proteins. Previous studies have indicated that Exo70 plays an important role in actin assembly and cell motility.

Immunogeno

GST-tagged recombinant protein corresponding to rat Exo70.

Applicazioni

Detect Exo70 using this Anti-Exo70 Antibody, clone 70X13F3 validated for use in Western Blotting, ICC.
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Western Blot Analysis: A 1:1,000 dilution from a representative lot detected Exo70 in 10 µg of human brain tissue lysate.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in Exo70 cells. (Hsu, S.C., et al. (2001). The Journal of Neuroscience. 21(11):3839–3848.)
Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF. (Hsu, S.C., et al. (2011). The Journal of Neuroscience. 31(10):3522–3535.)

Qualità

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Exo70 in 10 µg of rat brain tissue lysate.

Descrizione del bersaglio

~75 kDa observed

Stato fisico

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Rat brain tissue lysate

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Ilka Wilhelmi et al.
Molecular metabolism, 45, 101151-101151 (2020-12-29)
Hormone secretion from metabolically active tissues, such as pancreatic islets, is governed by specific and highly regulated signaling pathways. Defects in insulin secretion are among the major causes of diabetes. The molecular mechanisms underlying regulated insulin secretion are, however, not
Cristina Cacho-Navas et al.
Cellular and molecular life sciences : CMLS, 79(1), 61-61 (2022-01-10)
Apical localization of Intercellular Adhesion Receptor (ICAM)-1 regulates the adhesion and guidance of leukocytes across polarized epithelial barriers. Here, we investigate the molecular mechanisms that determine ICAM-1 localization into apical membrane domains of polarized hepatic epithelial cells, and their effect
Cristina Cacho-Navas et al.
eLife, 12 (2024-04-10)
Epithelial intercellular adhesion molecule (ICAM)-1 is apically polarized, interacts with, and guides leukocytes across epithelial barriers. Polarized hepatic epithelia organize their apical membrane domain into bile canaliculi and ducts, which are not accessible to circulating immune cells but that nevertheless

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