MABN378

Anti-Potassium Channel Kv10.1 Antibody, clone 56

clone 56, from mouse

• Sinonimo/i: Potassium voltage-gated channel subfamily H member 1, Ether-a-go-go potassium channel 1, EAG channel 1, h-eag, hEAG1, Voltage-gated potassium channel subunit Kv10.1, hEag1 Potassium channel, eag1
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 56, monoclonal
• Reattività contro le specie: rat, human
• tecniche: activity assay: suitable; immunofluorescence: suitable; immunohistochemistry: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_002229
• N° accesso UniProt: O95259
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... KCNH1(3756)

Descrizione

Descrizione generale

Potassium Channel Kv10.1 is also known as Potassium voltage-gated channel subfamily H member 1, Ether-a-go-go potassium channel 1 (EAG channel 1, h-eag, or hEAG1) and Voltage-gated potassium channel subunit Kv10.1. Potassium Channel Kv10.1 is a pore-forming (alpha) subunit of voltage-gated, non-inactivating, delayed rectifier potassium channel. Potassium Channel Kv10.1 mediates IK(NI) current in myoblasts, and can be modulated by cAMP as well as subunit assembly. Potassium Channel Kv10.1 is highly expressed in myoblasts at the onset of fusion and brain tissue. It is not found in normal epithelial cells, but has been detected in HeLa (cervical carcinoma), SH-SY5Y (neuroblastoma) and MCF-7 (epithelial tumor) cells.

Specificità

This antibody recognizes the extracellular, C-terminus domain of Potassium Channel Kv10.1. Additionally, this antibody does not cross react with hEag2 (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.).

Immunogeno

Epitope: Extracellular domain

Recombinant protein corresponding to the extracellular domain of human Potassium Channel Kv10.1.

Applicazioni

Detect Kv10.1 using this mouse monoclonal antibody, Anti-Potassium Channel Kv10.1 Antibody, clone 56 validated for use in IHC, Activity Assay & Immunofluorescence.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Potassium Channel Kv10.1 in human thalamus and cerebellum tissues.

Immunocytochemistry Analysis: A representative lot detected Potassium Channel Kv10.1 in transfected CHO-K1 and HEK293 cells (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.).

Activity Assay Analsyis: A representative lot from an independent laboratory inhibited anchorage-independent cancer cell growth in vitro (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.; Chen, Y., et al. (2011). PLoS One. 6(5):e19257.).

Activity Assay Analysis: A representative lot from independent laboratories inhibited Potassium Channel Kv10.1 currents in transfected HEK293 cells (Gomez-Varela, D., et al. (2007). Cancer Res. 67(15):7343-7349.).

Activity Assay Analysis: A representative lot from an independent laboratory inhibited cellular proliferation and
migration of certain Acute myeloid leukemia cell lines and primary cultured AML cells in vitro (Agarwal, J. R., et al. (2010). Mol Cancer. 9:18.).

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Qualità

Evaluated by Immunohistochemistry Analysis in rat frontal lobe tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Potassium Channel Kv10.1 in rat frontal lobe tissue.

Descrizione del bersaglio

111 kDa calculated

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Rat brain tissue

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable