MABE656

Anti-HP1γ Antibody, clone 14D3.1

clone 14D3.1, from mouse

• Sinonimo/i: Chromobox protein homolog 3, HECH, Heterochromatin protein 1 homolog gamma, HP1 gamma, Modifier 2 protein
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 14D3.1, monoclonal
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): rat (based on 100% sequence homology), mouse (based on 100% sequence homology), nonhuman primates (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG2aκ
• N° accesso NCBI: NP_009207
• N° accesso UniProt: Q13185
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CBX3(11335)

Descrizione

Descrizione generale

Chromobox protein homolog 3 (UniProt Q13185; also known as HECH, Heterochromatin protein 1 homolog gamma, HP1 gamma, Modifier 2 protein) is encoded by the CBX3 gene (Gene ID 11335) in human. HP1 is a non-histone chromosomal protein that is present as three orthologs, HP1-alpha, HP1-beta, and HP1-gamma, in mammals. While HP1-alpha and HP1-beta have been unambiguously localized in heterochromatin, HP1-gamma has been found to localize in both euchromatin and heterochromatin (transiently) compartments of interphase nuclei, as well as in the pericentromeric chromatin and arms of mitotic chromosomes of 3T3 cells. HP1-gamma is phosphorylated by PIM1 during interphase and may be hyper-phosphorylated during mitosis. HPI-gamma is involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at ′Lys-9′, leading to epigenetic repression. It may also contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). It is also reported to be involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Ref.: Minc E., et al. (2000). Cytogenet. Cell Genet. 90, 279-284.
Lachner, M., et al. (2001). Nature 410, 116-120.

Immunogeno

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human HP1γ.

Applicazioni

Anti-HP1γ, clone 14D3.1, Cat. No. MABE656, is a highly specific mouse monoclonal antibody that targets heterochromatin protein-1 gamma and has been tested in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected nuclear HP1γ immunoreactivity in 4% paraformaldehyde-fixed, 0.1 Triton X-100-permeabilized A431 and HeLa cells.

Research Category
Epigenetics & Nuclear Function

Qualità

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HP1γ in 10 µg of HeLa cell lysate.

Descrizione del bersaglio

~20 kDa observed. 20.68 kDa(Met1 removed) calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: MAB3450

Stato fisico

Format: Purified

Protein G purified.

Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1