MAB1905
Anti-Laminin α1/β 1 Antibody, clone AL-4
clone AL-4, Chemicon®, from rat
Sinonimo/i:
Laminin A Chain
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rat
Forma dell’anticorpo
affinity purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
AL-4, monoclonal
Reattività contro le specie
mouse
Produttore/marchio commerciale
Chemicon®
tecniche
ELISA: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG
N° accesso NCBI
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
mouse ... Lama1(16772)
Specificità
As original characterized, clone AL-4 reacts with EHS mouse laminin, A chain (laminin α1). The epitope is mapped to the carboxy terminal globular domain on the A chain. Recent work by Scheele et al (2006) has further demonstrated that clone AL-4, under reducing conditions, recognized both the 200 kDa β1/γ1 band and the 400 kDa α1 band of mouse EHS laminin. However the affinity for the 400 kDa band was very low. AL-4 also demonstrated strong binding the Laminin β1. By ELISA the epitope for the AL-4 seemed to be located on the Laminin β1 chain, however the clone also recognizes the laminin α1 chain in immunoblots, thus AL-4 seems to show mixed cross reactivity to laminins α1 and β1, with the β1 reactivity being more prominent.
Immunogeno
Laminin isolated from mouse EHS tumor.
Applicazioni
ELISA/RIA
Western blot
Immunoprecipitation
Immunohistochemistry. Reactive on tissue frozen in OCT at -70°C. Sections may be treated with hyaluronidase. Shows reactivity to paraffin embedded EHS tumor.
Optimal working dilutions must be determined by the end user.
Western blot
Immunoprecipitation
Immunohistochemistry. Reactive on tissue frozen in OCT at -70°C. Sections may be treated with hyaluronidase. Shows reactivity to paraffin embedded EHS tumor.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
This Anti-Laminin α1/β 1 Antibody, clone AL-4 is validated for use in ELISA, IP, WB, IH(P) for the detection of Laminin α1/β 1.
Stato fisico
Format: Purified
Purified from ascites by Protein G affinity chromatography. Purified immunoglobulin in 0.02M PB, 0.25M NaCl, pH=7.6, 0.1% sodium azide
Stoccaggio e stabilità
Maintain between 2 and 8°C.
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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