MAB1527
Anti-Peripherin Antibody, clone 8G2
culture supernatant, clone 8G2, Chemicon®
Sinonimo/i:
Anti-NEF4, Anti-PRPH1
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
100
300
Forma dell’anticorpo
culture supernatant
Tipo di anticorpo
primary antibodies
Clone
8G2, monoclonal
Reattività contro le specie
pig, rat, bovine, human, mouse
Produttore/marchio commerciale
Chemicon®
tecniche
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
Isotipo
IgG
Compatibilità
not suitable for immunohistochemistry (Paraffin)
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... PRPH2(5961)
Descrizione generale
Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems. Subsequently it was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig′s disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.
Specificità
Recognizes Peripherin. Strong staining on rat, mouse, human, pig and cow peripherin. Does not work on chicken or other more distantly related species, which may lack peripherin.
Immunogeno
Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990). 57:235-248] containing all but the 4 N-terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6M urea followed by preparative SDS-PAGE
Applicazioni
Anti-Peripherin Antibody, clone 8G2 is an antibody against Peripherin for use in IC, IH & WB.
Immunocytochemistry: 4% PFA fixed cells (5 minutes) or acetone is recommended.
Immunohistochemisty: light PFA fixation is necessary as 8G2 is sensitive to heavy formalin fixation. Antigen recovery is enhanced with 0.1% triton in the block only. 1:25-1:200
Immunoblotting: The antibody is clean and specific for the expected 57kDa band on Western blots.
Optimal working dilutions must be determined by end user.
Immunohistochemisty: light PFA fixation is necessary as 8G2 is sensitive to heavy formalin fixation. Antigen recovery is enhanced with 0.1% triton in the block only. 1:25-1:200
Immunoblotting: The antibody is clean and specific for the expected 57kDa band on Western blots.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Sensory & PNS
Neuronal & Glial Markers
Sensory & PNS
Neuronal & Glial Markers
Descrizione del bersaglio
57kDa
Stato fisico
UnPurified mouse culture supernatant containing no preservatives.
Unpurified
Stoccaggio e stabilità
Maintain for 12 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Risultati analitici
Control
Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate
Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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R C Larivière et al.
Journal of neurochemistry, 81(3), 525-532 (2002-06-18)
Peripherin is a type III intermediate filament (IF) abundantly expressed in developing neurons, but in the adult, it is primarily found in neurons extending to the peripheral nervous system. It has been suggested that peripherin may play a role in
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