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MAB10764

Sigma-Aldrich

Anti-SBP-tag Antibody, clone 20 | MAB10764

clone 20, 1.0 mg/mL, from mouse

Sinonimo/i:

streptavidin binding peptide

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100 μG
472,00 €

472,00 €


Spedizione prevista il28 aprile 2025Dettagli


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Cambia visualizzazione
100 μG
472,00 €

About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

472,00 €


Spedizione prevista il28 aprile 2025Dettagli


Richiedi un ordine bulk

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

20, monoclonal

Reattività contro le specie

human

Concentrazione

1.0 mg/mL

tecniche

immunocytochemistry: suitable
western blot: suitable

Isotipo

IgG1κ

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Streptavidin Binding Peptide tag is a useful and versatile peptide that can be inserted into expression vectors for easy detection of recombinant proteins and purification. The sequence for SBP-tag is 38 amino acids long having a nanomolar scale binding affinity for streptavidin with an equilibrium constant of 2.5 nM.

Specificità

Demonstrated to react with cells expressing the SBP tag.
This antibody recognizes SBP tag.

Immunogeno

Epitope: Unknown
Linear peptide corresponding to SBP tag.

Applicazioni

Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF. (Hudson, D., et al. (2008). MBC. 19(7):3070-3079.)
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Qualità

Evaluated by Western Blot in pTrAP-GFP transfected Hek293 lysate.

Western Blot Analysis: 0.1 ng/mL of this antibody detected SBP-tag on 10 µg of pTrAP-GFP transfected Hek293 lysate.

Descrizione del bersaglio

The protein expresss is pTrAP-GFP plasmids containing a Histidine tag, S-tag, streptavidin-binding pepide (SBP) tag, and GFP. The protein expressing is ~37 kDa

Stato fisico

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
pTrAP-GFP transfected Hek293 lysate

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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