MAB030
Anti-DNA Antibody, double stranded, clone BV16-13
culture supernatant, clone BV16-13, Chemicon®
Sinonimo/i:
Double-stranded DNA antibody
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Informazioni su questo articolo
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
BV16-13, monoclonal
Technique(s):
ELISA: suitable, immunocytochemistry: suitable, radioimmunoassay: suitable
Application:
ELISA, ICC, RIA
Citations:
30
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Servizio Tecnico
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Permettici di aiutartibiological source
mouse
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
BV16-13, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, radioimmunoassay: suitable
isotype
IgG2aκ
shipped in
wet ice
1 of 1
Questo articolo | |||
|---|---|---|---|
| clone BV16-13, monoclonal | clone JSB-1, monoclonal | clone P3 II-26, monoclonal | clone M2 I-4, monoclonal |
| antibody form culture supernatant | antibody form culture supernatant | antibody form culture supernatant | antibody form culture supernatant |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| Quality Level 100 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| isotype IgG2aκ | isotype IgG1 | isotype IgG2b | isotype IgG1 |
Immunogen
Epitope: double stranded, 28% reactive with single stranded
Application
Detect DNA with Anti-DNA Antibody, double stranded, clone BV16-13 (Mouse Monoclonal Antibody), that has been shown to work in ELISA, RIA, ICC.
EIA/RIA
Immunocytochemistry
Optimal working dilutions must be determined by end user.
Immunocytochemistry
Optimal working dilutions must be determined by end user.
Research Category
Epigenetics & Nuclear Function
Apoptosis & Cancer
Epigenetics & Nuclear Function
Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair
Apoptosis - Additional
Cell Cycle, DNA Replication & Repair
Apoptosis - Additional
Biochem/physiol Actions
Double stranded DNA (dsDNyA) (B-form) from human. Shows 95% reactivity to dsDNA and 28% reactivity to ssDNA.
BINDING ASSAY:
Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.
ANTIGEN BINDING
PROFILE: Relative Binding To:
Natural DNA Synthetic homopolymers
dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)
1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).
a) Native calf thymus DNA b) Heat-denatured calf thymus DNA
BINDING ASSAY:
Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation.
ANTIGEN BINDING
PROFILE: Relative Binding To:
Natural DNA Synthetic homopolymers
dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU)
1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01
Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0).
a) Native calf thymus DNA b) Heat-denatured calf thymus DNA
Physical form
Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ micron membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Classe di stoccaggio
10 - Combustible liquids
wgk
WGK 1
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The discovery that circulating nucleic acid-containing complexes in the serum of autoimmune lupus patients can stimulate B cells and plasmacytoid dendritic cells via Toll-like receptors 7 and 9 suggested that agents that block these receptors might be useful therapeutics. We
B Zhang et al.
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Contenuto correlato
Numero articolo commerciale globale
| SKU | GTIN |
|---|---|
| MAB030 | 04053252610851 |
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