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Sigma-Aldrich

Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11)

liquid, clone II-4C11, Calbiochem®

Sinonimo/i:

Collagen Type II antibody

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100 μG
566,00 €

566,00 €


Spedizione prevista il11 febbraio 2025Dettagli


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Cambia visualizzazione
100 μG
566,00 €

About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

566,00 €


Spedizione prevista il11 febbraio 2025Dettagli


Richiedi un ordine bulk

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

II-4C11, monoclonal

Stato

liquid

contiene

≤0.1% sodium azide as preservative

Reattività contro le specie

rat, human, bovine, rabbit

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze
avoid repeated freeze/thaw cycles

Isotipo

IgG1

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... COL2A1(1280)

Descrizione generale

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-X-63-Ag8-LUI mouse myeloma cells. Recognizes the ~130 kDa type II collagen protein.
Recognizes the ~130 kDa type II collagen protein. May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected.
This Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11) is validated for use in Frozen Sections for the detection of Collagen Type II (Ab-1).

Immunogeno

Human
purified collagen type II

Applicazioni

Frozen Sections (1-10 µg/ml)

Immunoblotting (1-5 µg/ml, see application references)

Paraffin Sections (1-10 µg/ml, see application references)

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C).

Altre note

Kumagai, J. et al. 1994 J. Anat.185, 278.
Kivrikko, K,I. 1993 Ann. Med.25, 113.
Sandell, L.J., et al. 1991 J. Cell. Biol.114, 1307.
Ryan, M.C., and Sandell, L.J. 1990 J. Biol. Chem.265, 10334.
Mayne, R., and Burgeson, R.E. 1987 Structure and Function of Collagen Types,Academic Press, Orlando, FL
May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected. This antibody may also react with other classes of collagen at a concentration higher than 1 µg/ml. In immunoblotting, a major band of ~130 kDa is obtained. Antibody should be titrated for optimal results in individual systems.

Note legali

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Shiping Shi et al.
Experimental and therapeutic medicine, 12(5), 3161-3166 (2016-11-25)
Osteoarthritis (OA) is a joint disease, and few treatments to date have been able to delay OA progression. The degradation of collagen type II (COL2) in the cartilage matrix is an important initiating factor for OA progression; the upregulation of
Han-Jun Kim et al.
Oxidative medicine and cellular longevity, 2020, 5915481-5915481 (2020-10-09)
Intervertebral disc degeneration (IVDD) is a common cause of lower back pain. Programmed cell death (PCD) including apoptosis and autophagy is known to play key mechanistic roles in the development of IVDD. We hypothesized that the nucleus pulposus cells that
En-Rung Chiang et al.
PloS one, 11(2), e0149835-e0149835 (2016-02-26)
Mesenchymal stem cell (MSC)-based therapies may aid in the repair of articular cartilage defects. The purpose of this study was to investigate the effects of intraarticular injection of allogeneic MSCs in an in vivo anterior cruciate ligament transection (ACLT) model
María Satué et al.
Scientific reports, 9(1), 10153-10153 (2019-07-14)
Intra-articular (IA) injection of mesenchymal stem cells (MSCs) promotes articular cartilage repair. However, cell fate and action after transplantation remain unclear. This study aimed at evaluating the biodistribution and efficacy of MSCs after IA injection. We used an immunocompetent, dual
En-Rung Chiang et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37(6), 1440-1450 (2019-05-08)
Intervertebral discs (IVDs) are important biomechanical components of the spine. Once degenerated, mesenchymal stem cell (MSC)-based therapies may aid in the repair of these discs. Although hypoxic preconditioning enhances the chondrogenic potential of MSCs, it is unknown whether bone marrow

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