ABS457

Anti-ASAH2 Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: N-acylsphingosine amidohydrolase, Non-lysosomal ceramidase 2, HNAC1, Neutral ceramidase, Acylsphingosine deacylase 2, ASAH2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• tecniche: western blot: suitable
• N° accesso UniProt: Q9NR71
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ASAH2(56624)

Descrizione

Descrizione generale

N-acylsphingosine amidohydrolase 2 (ASAH2), also known as neutral ceramidase (N-CDase, NCDase), is a key regulator of sphingolipid signaling metabolites. ASAH2 is known to hydrolyze the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Additionally, ASAH2 is suggested to act as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. ASAH2 is also suggested to be involved in the digestion of dietary sphingolipids in intestine tract. In this role, ASAH2 is implied to act as a key enzyme for the catabolism of dietary sphingolipids and consequently, regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. ASAH2 is expressed primarily in the intestine. It is also expressed with higher levels in kidney, skeletal muscle and heart.

Immunogeno

KLH-conjugated linear peptide corresponding to human ASAH2.

Applicazioni

Anti-ASAH2 Antibody is a highly specific rabbit polyclonal antibody, that targets ASAH2 & has been tested in western blotting.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Qualità

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected ASAH2 in 15 µg of HEK293 cell lysate.

Descrizione del bersaglio

~70 kDa observed. Uniprot describes 2 isoforms at ~82 kDa and ~86 kDa Uncharacterized bands may appear in some lysate(s).

Stato fisico

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
HEK293 cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable