AB15552
Anti-AT1 Receptor Antibody
Chemicon®, from rabbit
Sinonimo/i:
Angiotensin II receptory type 1, AGTR1
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rabbit
Livello qualitativo
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie
mouse, rat, human
Produttore/marchio commerciale
Chemicon®
tecniche
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... AGTR1(185)
Specificità
Recognizes AT1 Receptor (Angiotensin II receptory type 1, AGTR1).
The immunogen sequence is identical in canine.
Immunogeno
Purified peptide from the N-terminus of human AT1 Receptor (Accession number P30556).
Applicazioni
Anti-AT1 Receptor Antibody detects level of AT1 Receptor & has been published & validated for use in FC, IC, IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
CNS Control of Metabolism
Hormones & Receptors
CNS Control of Metabolism
Hormones & Receptors
Western blot: 1:200-1:500 using ECL on rat liver, rat kidney, mouse heart and mouse kidney lysate.
Immunohistochemistry on rat brain sections.
Immunocytochemistry: 1:100
Flow cytometry, indirect: 5-10 µg of antibody /1 x 106 cells
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on rat brain sections.
Immunocytochemistry: 1:100
Flow cytometry, indirect: 5-10 µg of antibody /1 x 106 cells
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Stato fisico
Format: Purified
ImmunoAffinity Purified
Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Stoccaggio e stabilità
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Risultati analitici
Control
Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 3
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
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Obesity has been associated with hepatic overexpression of the renin-angiotensin system (RAS). To evaluate the action of two angiotensin II (ANGII) receptor blockers (losartan or telmisartan) on the modulation of local hepatic RAS and the resulting metabolic effects in a
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Julius Benicky et al.
Cellular and molecular neurobiology, 32(8), 1353-1365 (2012-07-31)
Commercially available Angiotensin II AT1 receptor antibodies are widely employed for receptor localization and quantification, but they have not been adequately validated. In this study, six commercially available AT1 receptor antibodies were characterized by established criteria: sc-1173 and sc-579 from
Chien-Ning Hsu et al.
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