8.56044

Fmoc-Cys(Trt)-NovaSyn^® TGT

for peptide synthesis, Novabiochem^®

• Sinonimo/i: Trityl-Protected Cysteine Resin
• Codice UNSPSC: 12352209
• NACRES: NA.22

Proprietà

• Nome del prodotto: Fmoc-Cys(Trt)-NovaSyn^® TGT, Novabiochem^®
• Livello qualitativo: 200
• Nome Commerciale: NovaSyn^® TG; Novabiochem^®
• Stato: solid
• Impiego in reazioni chimiche: reaction type: Fmoc solid-phase peptide synthesis
• Produttore/marchio commerciale: Novabiochem^®
• applicazioni: peptide synthesis
• Gruppo funzionale: Fmoc
• Temperatura di conservazione: 15-25°C

Descrizione

Descrizione generale

Pre-loaded resin for synthesis of peptide acids and protected peptide fragments containing a C-terminal cysteine amino-acid residue by Fmoc SPPS. The base NovaSyn^® TG is a composite of low cross-linked polystyrene and 3000-4000 M.W. polyethylene glycol. Peptide synthesis is carried out at the ends of the PEG chains that have been functionalized with the hyper-acid labile 4-carboxytrityl alcohol linker. This use of this linker helps prevent racemization^[1] and β-piperidinylalanine^[2] formation during chain extension.^[3]Treatment of the peptidyl resin with 20% TFE in DCM or 1% TFA in DCM cleaves the product from the resin without affecting the standard TFA-labile side-chain protecting groups.^[4] Standard TFA cleavage releases the fully deprotected peptide.

Associated Protocols and Technical Articles:
Cleavage and Deprotection Protocols for Fmoc SPPS

Literature references:
^[1] E. Atherton, et al. in "Peptides 1990, Proc. 21st European Peptide Symposium, E. Giralt & D. Andreu(Eds), 1991, Escom, Leiden, pp. 243
^[2] J. Lukszo, et al. (1996) Lett. Pept. Sci., 3, 157.
^[3] Y. Fujiwara, et al. (1994) Chem. Pharm. Bull., 42, 724.
^[4] K. Barlos & D. Gatos in “Fmoc solid phase peptide synthesis: a practical approach”, W. C. Chan & P. D.White (Eds.), Oxford University Press, Oxford, 2000, pp. 218.

Applicazioni

Applications of Fmoc-Cys(Trt)-NovaSyn^® TGT include:

• the preparation of a phosphoprotein using Native Chemical Ligation (NCL).[5]
• the preparation of a peptide intermediate for use in glycoprotein semi-synthesis.[6]

Linkage

Replaces: 04-12-2705

Note legali

NOVASYN is a registered trademark of Merck KGaA, Darmstadt, Germany

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable