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71772

Millipore

ProteoExtract® Transmembrane Protein Extraction Kit

Sinonimo/i:

Membrane Protein Isolation Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali

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20 REACTIONS
499,00 €

499,00 €

Spedizione prevista il05 febbraio 2026Dettagli

Tutte le immagini(1)

Scegli un formato

Cambia visualizzazione
20 REACTIONS
499,00 €

About This Item

Codice UNSPSC:
41106500
NACRES:
NA.77

499,00 €

Spedizione prevista il05 febbraio 2026Dettagli

Produttore/marchio commerciale

Novagen®

Condizioni di stoccaggio

OK to freeze

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Descrizione generale

The ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).

Applicazioni

ProteoExtract® Transmembrane Protein Extraction Kit has been used: for extraction of protein from the apex segment of the colon in western blot analysis of Na+/H+ exchanger (NHE3)[1] for extracting plasma membrane proteins[2]

Caratteristiche e vantaggi

  • No sonication, intensive vortexing, lengthy ultracentrifugation, or high-temperature incubation is needed.
  • Reagents are sufficient to process a total of 40 samples.

Attenzione

Toxicity: Toxic (F)

Altre note

Nakamura, E., et al. 2008. Cancer Sci.99, 1390.

Note legali

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H315,H319

Classi di pericolo

Eye Irrit. 2 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Hepatic Tmem30a Deficiency Causes Intrahepatic Cholestasis by Impairing Expression and Localization of Bile Salt Transporters
Liu L, et al.
The American Journal of Pathology, 187(12), 2775-2787 (2017)
Impairment of electroneutral Na+ transport and associated downregulation of NHE3 contributes to the development of diarrhea following in vivo challenge with Brachyspira spp.
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The American Journal of Physiology, 318(2), G288?G297-G288?G297 (2020)
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The monocarboxylate transporters (MCTs) MCT1, MCT2, and MCT4 are essential components of the astrocyte-neuron lactate shuttle (ANLS), which is a fundamental element of brain energetics. Decreased expression of MCTs can induce cognitive dysfunction of the brain. In the present study
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Oncology reports, 42(3), 1237-1247 (2019-07-20)
Although cisplatin is one of the most accepted therapies for ovarian cancer, recurrence and drug resistance remain problematic. Both the ubiquitin‑proteasome system (UPS) and connexin (Cx) are closely related to tumor progression. However, the role of ubiquitin‑specific protease 14 (USP14) and Cx
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IIAEK (Ile-Ile-Ala-Glu-Lys, lactostatin) is a novel cholesterol-lowering pentapeptide derived from bovine milk β-lactoglobulin. However, the molecular mechanisms underlying the IIAEK-mediated suppression of intestinal cholesterol absorption are unknown. Therefore, we evaluated the effects of IIAEK on intestinal cholesterol metabolism in a
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