71353-M

Rosetta-gami 2(DE3)pLacI Competent Cells - Novagen

Escherichia coli, rod shaped

• Codice UNSPSC: 41106202
• NACRES: NA.81

Proprietà

• product name: Rosetta-gami 2(DE3)pLacI Competent Cells - Novagen, Rosetta-Gami 2 strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. Contains the pLacI plasmid producing extra Lac repressor.
• Origine biologica: Escherichia coli
• Livello qualitativo: 100
• Produttore/marchio commerciale: Novagen^®
• Condizioni di stoccaggio: OK to freeze
• Modalità di accrescimento: adherent or suspension
• Morfologia: rod shaped
• tecniche: microbiological culture: suitable
• Trasformazione cellulare: transformation efficiency: 2×10⁶ cfu/μg
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL (DE3) F′[lac⁺ lacI^q pro] gor522::Tn10 trxB pLacIRARE2 (Cam^R, Str^R, Tet^R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Expression host strains for pETBlue and pTriEx vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA.

Note that these strains are not recommended for use with pET, pACYCDuet, pCDF, pRSF, pCOLADuet or pETcoco vectors.

.Rosetta-gami 2 host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed in E. coli. These trxB/gor mutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs. In Rosetta-gami 2(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

Rosetta-Gami 2 strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. Contains the pLacI plasmid producing extra Lac repressor.

Componenti

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosetta-gami 2(DE3)pLacI Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Attenzione

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Note legali

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany