71346-M

Origami 2(DE3)pLysS Competent Cells - Novagen

Escherichia coli, rod shaped

• Sinonimo/i: Novagen Competent Cells
• Codice UNSPSC: 41106202
• NACRES: NA.81

Proprietà

• product name: Origami 2(DE3)pLysS Competent Cells - Novagen, Origami 2 strains have mutations in glutathione reducatase(gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation. T7 lysozyme expression suppresses basal T7 expression.
• Origine biologica: Escherichia coli
• Livello qualitativo: 100
• Produttore/marchio commerciale: Novagen^®
• Condizioni di stoccaggio: OK to freeze
• Modalità di accrescimento: adherent or suspension
• Morfologia: rod shaped
• tecniche: microbiological culture: suitable
• Trasformazione cellulare: transformation efficiency: 2×10⁶ cfu/μg
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C

Descrizione

Descrizione generale

Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139ahpC galE galK rpsL F′[lac⁺ lacI^q pro] (DE3) gor522::Tn10trxB pLysS (Cam^R, Str^R, Tet^R)





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Origami 2 strains have mutations in glutathione reducatase(gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation. T7 lysozyme expression suppresses basal T7 expression.

Origami 2 host strains are K-12derivatives that have mutations in both thethioredoxin reductase (trxB) and glutathionereductase (gor) genes, which greatly enhancedisulfide bond formation in the E. colicytoplasm. The Origami2 strains are kanamycin sensitive; like theoriginal Origami strains, the gor mutation is still selectedfor by tetracycline. To reduce the possibility ofdisulfide bond formation between molecules,strains containing mutations in trxB and gor arerecommended only for the expression of proteinsthat require disulfide bond formation for properfolding.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

Componenti

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlOrigami 2(DE3)pLysS Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Attenzione

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Note legali

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids