5.08940

G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested

• Sinonimo/i: G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested, G418, G 418
• Formula empirica (notazione di Hill): C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
• Numero CAS: 108321-42-2
• Peso molecolare: 692.71
• Numero MDL: MFCD00058314
• Codice UNSPSC: 12352200

Proprietà

• Saggio: ≥98% (TLC)
• Livello qualitativo: 200
• Forma fisica: liquid
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• Temperatura di conservazione: −20°C
• InChI: 1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1; UHEPSJJJMTWUCP-NKCAIAFTSA-N

Descrizione

Descrizione generale

Recommended reaction conditions: The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml

Confezionamento

Packaged under inert gas

Attenzione

Toxicity: Harmful (C)

Stato fisico

Sterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in 100 mM HEPES, pH 7.3.

Ricostituzione

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.

Altre note

Ethier, S.P., and Taback, E. 1993. Cancer Lett.74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol.7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol.133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol.163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun.101, 1031.
Jimenez, A., and Davies, J. 1980. Nature287, 869.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany