07-1364

Anti-ECT2 Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: epithelial cell transforming sequence 2 oncogene, Epithelial cell-transforming sequence 2 oncogene, protein ECT2
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: pig, human
• Reattività contro le specie (prevista in base all’omologia): porcine (based on 100% sequence homology), rabbit (based on 100% sequence homology), monkey (based on 100% sequence homology), opossum (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable; western blot: suitable
• N° accesso UniProt: Q9H8V3
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ECT2(1894)

Descrizione

Descrizione generale

Epithelial cell-transforming sequence 2 oncogene (ECT2) is a guanine nucleotide exchange factor or GEF that belongs to the Dbl family of Rho GEFs. ECT2 is composed of 2 BRCT domains within the N-terminus, a DBL-homology domain, and a PH domain. Studies have shown ECT2 to localize to both the cell cortex and central spindles, and that it is a crucial part of cleavage furrow formation during cytokenises. Overexpression of cleaved ECT2, containing only the N-terminal domain, has been shown to cause multinucleation. As a GEF it has been shown to bind specifically to Rac proteins, RhoA, and RhoC.

Specificità

This antibody recognizes ECT2 at the
C-terminus.

Immunogeno

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to human ECT2 at the C-terminus.

Applicazioni

Anti-ECT2 Antibody is an antibody against ECT2 for use in WB & IC.

Research Category
Signaling

Research Sub Category
G-proteins

Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected ECT2 in A549 cell lysate.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory on Heya8 cells. (Der Channing, University of North Carolina at Chapel Hill, NC, channing_der@med.unc.edu)

Qualità

Evaluated by Western Blot in A549 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected ECT2 on 10 µg of A549 cell lysate.

Descrizione del bersaglio

~ 105 kDa observed

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
A549 cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable