06-896
Anti-GFP (Green Fluorescent Protein) Antibody
Upstate®, from chicken
Sinonimo/i:
Green fluorescent protein
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200 μG
653,00 €
653,00 €
Spedizione prevista il18 aprile 2025Dettagli
Per il tuo target è disponibile un anticorpo ricombinante privo di conservanti. Prova ZRB1097
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Scegli un formato
Cambia visualizzazione
200 μG
653,00 €
About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
653,00 €
Spedizione prevista il18 aprile 2025Dettagli
Per il tuo target è disponibile un anticorpo ricombinante privo di conservanti. Prova ZRB1097
Richiedi un ordine bulk
Prodotti consigliati
Origine biologica
chicken
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Reattività contro le specie
vertebrates
Produttore/marchio commerciale
Upstate®
tecniche
immunocytochemistry: suitable
western blot: suitable
Isotipo
IgY
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Descrizione generale
Green fluorescent protein (GFP) is a 29 kDa fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488 nm), GFP strongly fluoresces (Emission max: 507 nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells.
Specificità
Recognizes green fluorescent protein (GFP), Mr 30 kDa, and GFP-fusion proteins.
Immunogeno
His-tagged green fluorescent protein of Aequorea victoria.
Applicazioni
Anti-GFP (Green Fluorescent Protein) Antibody detects level of GFP (Green Fluorescent Protein) & has been published & validated for use in IC & WB.
Immunocytochemistry:
This antibody was reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein.
This antibody was reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Qualità
Routinely evaluated by Western Blot on rat brain lysate containing purified GFP (Catalog #14-392) or a GFP-fusion protein from a transfected E. coli cell lysate.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected Green Fluorescent Protein from 50 μg of rat brain lysate containing 25 ng of purified Green Fluorescent Protein (Catalog # 14-392). A previous lot of this antibody also detected a GFP-fusion protein from a transfected E. coli cell lysate.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected Green Fluorescent Protein from 50 μg of rat brain lysate containing 25 ng of purified Green Fluorescent Protein (Catalog # 14-392). A previous lot of this antibody also detected a GFP-fusion protein from a transfected E. coli cell lysate.
Descrizione del bersaglio
30 kDa
Stato fisico
Ammonium Sulfate Precipitation and PEG
Format: Purified
Purified chicken polyclonal IgY from egg yolk in buffer containing 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.
Stoccaggio e stabilità
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Risultati analitici
Control
Rat brain lysate, cells expressing GFP protein.
Rat brain lysate, cells expressing GFP protein.
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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