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Merck
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Documenti fondamentali

06-157

Sigma-Aldrich

Anti-RasGAP Antibody

Upstate®, from rabbit

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

rabbit

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Reattività contro le specie

human, rat, mouse, avian

Produttore/marchio commerciale

Upstate®

tecniche

immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... TSR3(115939)

Specificità

GAP

Immunogeno

Trp E fusion corresponding to residues 171-448 of human RasGAP

Applicazioni

Anti-RasGAP Antibody is an antibody against RasGAP for use in IP & WB.
Research Category
Signaling
Research Sub Category
MAP Kinases

Qualità

Western Immunoblot Analysis: This antibody, at 0.5 and 2.0 μg/ml, recognized the 120 kDa GAP protein in a RIPA lysate of human Raji lymphocyte cells, lysates of rat L6 myoblasts and mouse Wehi leukemia cells.
Immunoprecipitation: 5 μg of this antibody immunoprecipitated the 120kDa GAP protein from a RIPA lysate of human Raji lymphocyte cells.

Descrizione del bersaglio

120kDa

Linkage

Replaces: 04-577; 04-1039

Stato fisico

200 μg of protein-A purified IgG. Lyophilized from 200 μl of 0.1 M Tris-HCl, 0.15 M NaCl, and 0.05% Na Azide.
Format: Purified
Protein A chromatography

Stoccaggio e stabilità

Lyophilized: 2 years at -20°C; Rehydrated: 6 months at -20°C Rehydrate in 200 μl of water.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pittogrammi

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Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1


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S J Ruff et al.
The Journal of biological chemistry, 272(2), 1263-1267 (1997-01-10)
The intraperitoneal injection of a vanadate/H2O2 mixture (peroxovanadate) into mice resulted within minutes in the appearance of numerous tyrosine-phosphorylated proteins in the liver and kidney. These effects are presumably due to the inhibition of phosphotyrosine phosphatase activity. Three of the
F Authier et al.
The Biochemical journal, 319 ( Pt 1), 149-158 (1996-10-01)
We have investigated the biosynthesis, subcellular location and expression of insulin-degrading enzyme (IDE). a type-I peroxisomal protease, in semi-permeabilized hepatoma cells using pulse-chase experiments, non-denaturing immunoprecipitation protocols and Northern-blot analyses. In HcpG2 cell lysates prepared from cells radiolabelled with Tran[35S]-label
A conserved region of c-Ha-Ras is required for efficient GTPase stimulation by GTPase activating protein but not neurofibromin
Yoder-Hill, J., et al
The Journal of Biological Chemistry, 270, 27615-27621 (1995)
P J Owen-Lynch et al.
The Journal of biological chemistry, 270(11), 5956-5962 (1995-03-17)
A temperature-sensitive mutant of the v-Abl protein has previously been shown to exhibit tyrosine protein kinase activity in Interleukin 3 (IL-3)-dependent IC.DP cells grown at the permissive temperature (32 degrees C) but not at the restrictive temperature (39 degrees C).
An effector-like function of Ras GTPase-activating protein predominates in cardiac muscle cells
Abdellatif, M. and Schneider, M. D.
The Journal of Biological Chemistry, 272, 525-533 (1997)

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