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Documenti fondamentali

06-1328

Sigma-Aldrich

Anti-Themis Antibody

from rabbit, purified by affinity chromatography

Sinonimo/i:

thymocyte selection associated, thymocyte selection pathway associated, chromosome 6 open reading frame 190, chromosome 6 open reading frame 207, protein THEMIS, Thymocyte-expressed molecule involved in selection

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100 μG
416,00 €

416,00 €

Spedizione prevista il03 marzo 2025Dettagli

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100 μG
416,00 €

About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

416,00 €

Spedizione prevista il03 marzo 2025Dettagli

Richiedi un ordine bulk

Origine biologica

rabbit

Livello qualitativo

100

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Purificato mediante

affinity chromatography

Reattività contro le specie

mouse

Reattività contro le specie (prevista in base all’omologia)

rat (73% sequence homology)

tecniche

flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

N° accesso NCBI

NP_848781

N° accesso UniProt

Q8BGW0

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

mouse ... Themis(210757)

Categorie correlate

Descrizione generale

Thymocyte-expressed molecule involved in selection (Themis) is a highly conserved protein that has been shown to serve a key role in regulating T-cell development through TCR signaling, especially through the moderation of phosphorylation and calcium influx. It is also a critical component in the later stages of thymocyte development through the regulation of positive/negative T-cell selection, and is required for integration and maintenance of signals necessary to T-cell lineage commitment and maturation. Themis expression is observed in thymus, spleen, thymocytes between Pre-TCR and checkpoints positive in selectin, and at low levels in mature T-cells.

Specificità

This antibody recognizes Themis at the C-terminus.

Immunogeno

KLH-conjugated linear peptide corresponding to the C-terminus of mouse Themis.

Applicazioni

Detect Themis using this Anti-Themis Antibody validated for use in WB, FC.

Qualità

Evaluated by Western Blot in mouse thymocyte tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Themis in 10 µg of mouse thymocyte tissue lysate.

Descrizione del bersaglio

~72 kDa observed.
UniProtKB/Swiss-Prot Q8BGW0 (THMS1_MOUSE) entry describes 5 isoforms produced by alternative splicing: Isoform 1 at 72.780 kDa, Isoform 2 at 69.544 kDa, Isoform 3 at 68.293 kDa, Isoform 4 at 59.082 kDa, and Isoform 5 at 30.040 kDa

Risultati analitici

Control
Mouse thymocyte tissue lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Mukul Prasad et al.
Cellular & molecular immunology, 18(9), 2249-2261 (2020-11-13)
Themis is a T cell lineage-specific molecule that is involved in TCR signal transduction. The effects of germline Themis deletion on peripheral CD4+ T cell function have not been described before. In this study, we found that Themis-deficient CD4+ T
Namrata Gautam et al.
Life science alliance, 6(12) (2023-09-23)
Themis is important in regulating positive selection of thymocytes during T cell development, but its role in peripheral T cells is less understood. Here, we investigated T cell activation and its sequelae using a tamoxifen-mediated, acute Themis deletion mouse model.
Themis sets the signal threshold for positive and negative selection in T-cell development.
Fu, G; Casas, J; Rigaud, S; Rybakin, V; Lambolez, F; Brzostek, J; Hoerter, JA; Paster et al.
Nature null
Joanna Brzostek et al.
Nature immunology, 21(2), 186-198 (2020-01-15)
T cell homeostasis and functional responsiveness require signals from self-peptide-major histocompatibility complex (self-pMHC) and cytokines, but the mechanisms controlling this signal integration are unknown. Using a conditional deletion of the T cell lineage-specific protein Themis, we show that Themis is
Differential function of Themis CABIT domains during T cell development.
Okada, T; Nitta, T; Kaji, K; Takashima, A; Oda, H; Tamehiro, N; Goto, M; Okamura et al.
Testing null

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