Dye content is determined by back calculation from the carbon or nitrogen content, while purity is established by HPLC. The dye content is 60%. The impurities are not identified by either dye content or by methods such as HPLC, and no testing for impurities is conducted in this case. Both dye content and dye purity are determined for this product, with the Specification sheet listing minimum specifications for all lots, and individual Certificates of Analysis reporting lot-specific results. Water content is not measured, and the product is not considered hygroscopic.
302643
Trypan Blue
Dye content 60 %, ≥80% (HPLC)
Sinonimo/i:
Direct blue 14
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25 G
78,20 €
100 G
260,00 €
About This Item
Formula condensata:
C34H24N6O14S4Na4
Numero CAS:
Peso molecolare:
960.81
Colour Index:
23850
Beilstein:
4360496
Numero CE:
Numero MDL:
Codice UNSPSC:
12171500
ID PubChem:
NACRES:
NA.47
Prodotti consigliati
Livello qualitativo
Saggio
≥80% (HPLC)
Stato
powder
Composizione
Dye content, 60%
tecniche
microbe id | staining: suitable
Colore
dark green to brown, or Brown-Black
Punto di fusione
>300 °C (lit.)
Solubilità
H2O: 1 mg/mL
λmax
607 nm
ε (coefficiente d’estinzione)
≥60000 at 603-607 nm in methanol at 0.01 g/L
60000 at 603-607 nm in methanol
applicazioni
diagnostic assay manufacturing
hematology
histology
Temperatura di conservazione
room temp
Stringa SMILE
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
GLNADSQYFUSGOU-UHFFFAOYSA-J
Informazioni sul gene
human ... PTPN1(5770) , PTPRF(5792)
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Descrizione generale
Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B.
Applicazioni
Azioni biochim/fisiol
Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.
This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.
This dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.
Trypan blue is routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains. It is suitable for use in cell viability assay:
- to determine the number of living and necrotic cells by staining with 0.2% trypan blue solution.
- to stain plant tissue in order to visualize cell death.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Carc. 1B
Codice della classe di stoccaggio
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Classe di pericolosità dell'acqua (WGK)
WGK 3
Dispositivi di protezione individuale
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
Elenchi normativi
Forniamo informazioni su eventuali restrizioni prevalentemente per i prodotti chimici. Per altre tipologie di prodotto siamo in grado di fornire soltanto informazioni limitate. Nessuna segnalazione significa che nessuno dei componenti è citato in un elenco. È dovere dell’utilizzatore assicurarsi che il prodotto venga impiegato in maniera sicura e a norme di legge.
EU REACH Annex XVII (Restriction List)
CAS No.
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Are the descriptions of Product 302643 as "Trypan Blue - 60% Dye content" and the purity limit of ">= 80.0%" on the Specification sheet and CofA referring to the same thing? If they are, why are they different? If not, should both be considered when aiming to achieve a specific dye concentration? Additionally, if it is 60% dye, what makes up the remaining 40%? Should the significant water content in this product be taken into consideration?
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