RCP

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC kód: 41106514

Tulajdonságok

• biológiai forrás: rat brain
• kiszerelés: tube of 5 μg 00031626-DNA-5UG; pkg of vial of cells 00031626-1VL
• növekedési mód: Adherent
• kariotípus: Not specified
• morfológia: Endothelial
• termékek: Not specified
• receptorok: Vasopressin V1a, Angiotensin II, Endothelin 1, Bradykinin, Serotonin
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Alkalmazás

Toxicology, cellular signalling and pharmacology studies

Biokémiai/fiziológiai hatások

Rat Choroid Plexus

The choroid plexus of adult Wistar Rats were dissected from the fourth ventricle to give a primary culture. This primary culture was then immortalized by transfection with the TSori minus adenovirus (a replication deficient adenovirus containing SV40 large T antigen). Sub-cloning of the immortalised cells was performed by selecting for clones which exhibited calcium ion release in response to vasopressin, angiotensin, bradykinin, serotonin or endothelin stimulation. The sub-clone which exhibited the largest response to all five hormones was selected and sub-cultured on to become RCP.

Elkészítési megjegyzés

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 0.1% Epidermal Growth Factor(EGF) (E9644), 1% Insulin–Transferrin–Selenium(ITS) (I3146)

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Egyéb megjegyzések

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