Összes fotó(1)
Fontos dokumentumok
11965085001
Roche
Anti-His6-Peroxidase
from mouse IgG1
Szinonimák:
antibody
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
klón:
BMG-his-1, monoclonal
application:
citations:
16
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
konjugátum
peroxidase conjugate
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
BMG-his-1, monoclonal
Forma
lyophilized
kiszerelés
pkg of 50 U
gyártó/kereskedő neve
Roche
izotípus
IgG1
tárolási hőmérséklet
2-8°C
Általános leírás
Anti-His6-Peroxidase is a monoclonal antibody to His6-tagged proteins, conjugated to horseradish peroxidase. Anti-His6-Peroxidase specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the epitope-sequence location; however, it preferentially recognizes the C-terminal His6 epitope with high sensitivity.
Egyediség
Anti-His6-Peroxidase specifically recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the epitopesequence. However, Anti-His6-Peroxidase preferentially recognizes the C-terminal His6 epitope with high sensitivity. Anti-His6 allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used.
The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.
Alkalmazás
Anti-His6-Peroxidase has been used in in vitro serum stability of fusion toxins, pull-down assay and western blotting.
Anti-His6-Peroxidase is used for the detection of His6-tagged proteins in:
- ELISA (enzyme-linked immunosorbent assay)
- Western blot
Minőség
Function test: Western blot using extracts from cell line expressing a recombinant His6-tagged protein.
Elkészítési megjegyzés
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
The following concentrations should be taken as a guideline:
- ELISA: 100 mU/ml
- Western blot: 100mU/ml
Feloldás
Add 1 ml double-distilled water to a final concentration of 50 U/ml.
Reconstitution should be performed for at least 10 minutes.
Reconstitution should be performed for at least 10 minutes.
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
Nem találja a megfelelő terméket?
Próbálja ki a Termékválasztó eszköz. eszközt
Figyelmeztetés
Warning
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Skin Sens. 1
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 1
Lobbanási pont (F)
does not flash
Lobbanási pont (C)
does not flash
Válasszon a legfrissebb verziók közül:
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Az ügyfelek ezeket is megtekintették
Identification and Characterization of Heptaprenylglyceryl Phosphate Processing Enzymes in Bacillus subtilis
Linde M, et al.
The Journal of Biological Chemistry, 291(28), 14861-14870 (2016)
Clare Sheridan et al.
Molecular cell, 31(4), 570-585 (2008-08-30)
Bax and Bak promote apoptosis by perturbing the permeability of the mitochondrial outer membrane and facilitating the release of cytochrome c by a mechanism that is still poorly defined. During apoptosis, Bax and Bak also promote fragmentation of the mitochondrial
Dinesh Adhikary et al.
The Journal of experimental medicine, 203(4), 995-1006 (2006-03-22)
Epstein-Barr virus (EBV) establishes lifelong persistent infections in humans by latently infecting B cells, with occasional cycles of reactivation, virus production, and reinfection. Protective immunity against EBV is mediated by T cells, but the role of EBV-specific T helper (Th)
KaiC intersubunit communication facilitates robustness of circadian rhythms in cyanobacteria
Kitayama Y, et al.
Nature Communications, 4 (2013)
Elaheh Gheybi et al.
Cancer reports (Hoboken, N.J.), 6(3), e1745-e1745 (2022-10-28)
CD44, as a tumor-associated marker, can be used to detect stem cells in breast cancer. While CD44 is expressed in normal epithelial cells, carcinoma cells overexpress CD44. In the current study, we designed a recombinant protein that included the variable
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással