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Product Name
AC2, 86061902
biological source
mouse blood
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Interleukin 3 (IL-3)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse lymphoblast
Cell Line Description
Derived from a mouse basophil clone and is dependent on Interleukin-3 (IL-3) for growth. IL-3 may be added in the form of Wehi conditioned media (10-20%). Culture densities between 10,000 and 100,000 cells/ml is necessary to retain cells in exponential growth phase.
Application
Growth factor studies, IL-2 assays
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 10,000 to 100,000 cells/ml; 5% CO2; 37°C. Cells will rapidly die at high densities above 5x100,000 cells/ml. Culture very sensitive to agitation.
Other Notes
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