Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

View All Documentation

N3786

Sigma-Aldrich

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

Proteomics Grade, suitable for MALDI-TOF MS

Sinónimos:

Neuraminidase from Arthrobacter ureafaciens, Acyl-neuraminyl Hydrolase, Sialidase

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

Número de CAS:
9001-67-6
Comisión internacional de enzimas:
3.2.1.18 (BRENDA, IUBMB)
EC Number:
232-624-6
MDL number:
MFCD00131711
UNSPSC Code:
12352204
NACRES:
NA.32

form

lyophilized powder

Quality Level

200

quality

Proteomics Grade

specific activity

≥25 U/vial

suitability

suitable for MALDI-TOF MS

storage temp.

2-8°C

¿Está buscando productos similares? Visita Guía de comparación de productos

Application

Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) linkages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein.

Packaging

Provided with 5× concentrate reaction buffer.

Quality

The buffer solution has been specifically formulated to assure maximum enzyme stability and assay sensitivity.

Unit Definition

One unit will release 1 nmole of 4-methylumbelliferone from 2-(4-methylumbelliferyl) α-D-N-acetylneuraminic acid per minute at pH 5.5 at 37° C.

Physical form

Lyophilized powder

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • N3536Neuraminidase 5X Reaction Buffer 1.5 mLSDS

related product

Referencia del producto
Descripción
Precios

P2613

ProteoMass™ P14R MALDI-MS Standard, vial of 10 nmol

C8857

Citocromo c from equine heart, vial of 10 nmol, (M+H+) 12,361.96 Da by calculation

C8982

α-Cyano-4-hydroxycinnamic acid, suitable for MALDI-TOF MS

A8346

ProteoMass™ ACTH Fragment 18-39 MALDI-MS Standard, vial of 10 nmol, monoisotopic mol wt 2,464.1989 Da

I6154

ProteoMass™ Insulin chain B oxidized MALDI-MS Standard, vial of 10 nmol

I6279

Insulina from bovine pancreas, vial of 10 nmol, monoisotopic mol wt 5,729.6087 Da

A8471

ProteoMass™ Albumin MALDI-MS Standard, vial of 10 nmol, average mol wt 66,429.09 Da by calculation

A9096

ProteoMass™ Aldolase MALDI-MS Standard, vial of 10 nmol, average mol wt 39,211.28 Da by calculation

A8846

ProteoMass™ Angiotensin II MALDI-MS Standard, vial of 10 nmol

A8971

ProteoMass™ Apomyoglobin MALDI-MS Standard, vial of 10 nmol, (M+H+) 16,952.27 Da by calculation

B4181

ProteoMass™ Bradykinin Fragment 1-7 MALDI-MS Standard, vial of 10 nmol, monoisotopic mol wt 756.3997 Da

MSCAL1

ProteoMass™ Peptide and Protein MALDI-MS Calibration Kit, Peptide and Protein MALDI MS Calibration Standard

MSCAL2

ProteoMass™ Peptide MALDI-MS Calibration Kit, Peptide MALDI MS Calibration Standard

MSCAL3

ProteoMass™ Protein MALDI-MS Calibration Kit, Protein MALDI MS Calibration Standard

56197

3-Hydroxypicolinic acid, matrix substance for MALDI-MS, ≥99.0% (HPLC)

54793

2-(4-Hydroxyphenylazo)benzoic acid, matrix substance for MALDI-MS, ≥99.5%

85429

Sinapic acid, matrix substance for MALDI-MS, ≥99.0% (T)

03565

MALDI validation set polystyrene Mp ~700 - 70 000, certified reference material

37468

2′,6′-Dihydroxyacetophenone, matrix substance for MALDI-MS, ≥99.5%

91928

2′,4′,6′-Trihydroxyacetophenone monohydrate, matrix substance for MALDI-MS, ≥99.5%

03597

MALDI validation set PS, PMMA, PDMS, PEG and PSS Mp ~4′300 - 6′500

87884

trans-2-[3-(4-tert-Butylphenyl)-2-methyl-2-propenylidene]malononitrile, matrix substance for MALDI-MS, ≥99.0% (HPLC)

60018

Caffeic acid, matrix substance for MALDI-MS, ≥99.0% (HPLC)

29208

(N-Succinimidyloxycarbonylmethyl)tris(2,4,6-trimethoxyphenyl)phosphonium bromide, for protein sequence analysis (by MALDI-MS), ≥98.5%

46278

Ferulic acid, matrix substance for MALDI-MS, ≥99.0% (HPLC)

85707

2,5-Dihydroxybenzoic acid, matrix substance for MALDI-MS, >99.0% (HPLC)

70990

α-Cyano-4-hydroxycinnamic acid, matrix substance for MALDI-MS, ≥99.0% (HPLC)

39468

α-Cyano-4-hydroxycinnamic acid, matrix substance for MALDI-MS, Ultra pure

39319

2,5-Dihydroxybenzoic acid, matrix substance for MALDI-MS, ≥99.5% (HPLC), Ultra pure

49508

Sinapic acid, matrix substance for MALDI-MS, ≥99.5%, Ultra pure

50862

Super-DHB, suitable for matrix substance for MALDI-MS, ≥99.0%

02241

2′,3′-Dideoxycytidine 5′-triphosphate sodium salt solution, suitable for MALDI-MS, ≥98.0% (HPLC)

42597

3′-Deoxythymidine 5′-triphosphate sodium salt solution, BioChemika, puriss. p.a., suitable for MALDI-MS, ≥98.0% (HPLC)

MS0100

ProteoMass™ Guanidination Kit, For improving MALDI-MS sensitivity and peptide sequence coverage

MSCAL4

ProteoMass™ MALDI Calibration Kit for Exactive Series MS, LTQ XL and LTQ Hybrids, MALDI MS Calibration Standard

92817

9-Aminoacridine, matrix substance for MALDI-MS, ≥99.5% (HPLC)

56451

1,5-Diaminonaphthalene, matrix substance for MALDI-MS, ≥99.0% (HPLC)

50757

Micro-Array for Mass Spectrometry (MAMS), for single cell analysis

94141

4-Chloro-α-cyanocinnamic acid, matrix substance for MALDI-MS, ≥95.0% (HPLC)

89063

4-Bromo-α-cyanocinnamic acid, matrix substance for MALDI-MS, ≥95.0% (HPLC)

77081

α-Cyano-4-fluorocinnamic acid, matrix substance for MALDI-MS, ≥95.0% (HPLC)

38419

α-Cyano-2,3,4,5,6-pentafluorocinnamic acid, matrix substance for MALDI-MS, ≥95.0% (HPLC)

39379

4-Chloro-α-cyanocinnamic acid - α-Cyano-2,4-difluorocinnamic acid mixture, matrix substance for MALDI-MS, ≥95.0% (sum of both components, HPLC)

68914

4-Bromo-α-cyanocinnamic acid - 4-Chloro-α-cyanocinnamic acid mixture, matrix substance for MALDI-MS, ≥95.0% (sum of both components, HPLC)

05851

4-Aminoquinaldine, matrix substance for MALDI-MS, ≥99.0% (HPLC)

78246

Curcumin, matrix substance for MALDI-MS, ≥99.5% (HPLC)

04973

Micro-Array for Mass Spectrometry (MAMS), for quantitative MALDI

94477

(E)-2-Cyano-3-(2-naphthyl)acrylic acid, matrix substance for MALDI-MS, ≥98.0% (HPLC)

83788

(2E)-3-(9-Anthryl)-2-cyanoacrylic acid, matrix substance for MALDI-MS, ≥97.0% (HPLC)

76884

Anthranilamide, matrix substance for MALDI-MS, ≥99.0% (HPLC)

56229

9-Nitroanthracene, matrix substance for MALDI-MS, ≥98.5% (HPLC)

PFS11

SpheriCal™ Mix, Peptide Low, suitable for MALDI-MS

PFS31

SpheriCal™ Aqua, Peptide Medium, suitable for MALDI-MS

PFS32

SpheriCal™ Aqua, Peptide High, suitable for MALDI-MS

PFS20

SpheriCal™ Neat, Peptide Low, suitable for MALDI-MS

PFS21

SpheriCal™ Neat, Peptide Medium, suitable for MALDI-MS

PFS22

SpheriCal™ Neat, Peptide High, suitable for MALDI-MS

PFS23

SpheriCal™ Neat, Protein Low, suitable for MALDI-MS

PFS30

SpheriCal™ Aqua, Peptide Low, suitable for MALDI-MS

69028

4-Phenyl-α-cyanocinnamamide, matrix substance for MALDI-MS, ≥98.5% (HPLC)

900666

Bruker standard solvent, suitable for MALDI-MS, 50% ACN + 47.5% H2O + 2.5% TFA

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Natallia Makarava et al.
Acta neuropathologica communications, 6(1), 92-92 (2018-09-14)
Last decade witnessed an enormous progress in generating authentic infectious prions or PrPSc in vitro using recombinant prion protein (rPrP). Previous work established that rPrP that lacks posttranslational modification is able to support replication of highly infectious PrPSc with assistance
Hongyun Li et al.
PloS one, 11(2), e0148238-e0148238 (2016-02-02)
Recent studies have shown that cerebral apoD levels increase with age and in Alzheimer's disease (AD). In addition, loss of cerebral apoD in the mouse increases sensitivity to lipid peroxidation and accelerates AD pathology. Very little data are available, however
Patricia J Campbell et al.
Journal of virology, 88(7), 3802-3814 (2014-01-17)
The 2009 H1N1 lineage represented the first detection of a novel, highly transmissible influenza A virus genotype: six gene segments originated from the North American triple-reassortant swine lineage, and two segments, NA and M, derived from the Eurasian avian-like swine
Tadatsugu Imamura et al.
Journal of virology, 88(5), 2374-2384 (2013-12-29)
Increased detection of enterovirus 68 (EV68) among patients with acute respiratory infections has been reported from different parts of the world in the late 2000s since its first detection in pediatric patients with lower-respiratory-tract infections in 1962. However, the underlying
Alana L Woodward et al.
Veterinary microbiology, 169(3-4), 113-127 (2014-02-01)
Equine influenza viruses are a major cause of respiratory disease in horses worldwide and undergo antigenic drift. Several outbreaks of equine influenza occurred worldwide during 2010-2012, including in vaccinated animals, highlighting the importance of surveillance and virus characterisation. Virus isolates
Ver todos los artículos relacionados

Artículos

Glycan Sequencing Using Exoglycosidases

Glycan Sequencing Using Exoglycosidases

Glycan Sequencing Using Exoglycosidases

Glycan Sequencing Using Exoglycosidases

Glycan Sequencing Using Exoglycosidases

Glycan Sequencing Using Exoglycosidases

Glycan Sequencing Using Exoglycosidases

Glycan Sequencing Using Exoglycosidases

Ver todo

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico