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Merck

MS0100

Supelco

ProteoMass Guanidination Kit

For improving MALDI-MS sensitivity and peptide sequence coverage

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.24

Quality Level

product line

ProteoMass

application(s)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

compatibility

for use with (Complex cell extracts, pure protein solution, 1D and 2D PAGE gels)

Application

The ProteoMass Guanidination Kit allows you to enhance MALDI-MS sensitivity, increase sequence coverage, and identify peptides with greater confidence. Following proteolytic digestion, peptides with C-terminal Arginine residues are ionized preferentially over peptides with C-terminal Lysine residues, leading to compromised sequence coverage and limited confidence during peptide mass fingerprint analysis. The ProteoMass Guanidination Kit efficiently and conveniently converts C-terminal Lysine residues to homoarginine, increasing MALDI signal strength and producing enhanced sequence coverage.

Features and Benefits

  • Identify more samples with greater accuracy and confidence
  • Increase throughput and save time - only 35 minutes to use the kit vs. 2 hours using traditional methods
  • Compatibility - compatible with 1D or 2D PAGE gel bands or spots, as well as complex cell extracts

Other Notes

One MS0100 kit is sufficient for 96 reactions.

Legal Information

ProteoMass is a trademark of Sigma-Aldrich Co. LLC

Related product

Referencia del producto
Descripción
Precios

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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A variety of N-glycans attached to protein are known to involve in many important biological functions. Endoplasmic reticulum (ER) and Golgi localized enzymes are responsible to this template-independent glycan synthesis resulting glycoforms at each asparagine residues. The regulation mechanism such
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Group B Streptococcus pili are covalently linked structures assembled via a sortase-catalyzed transpeptidation mechanism involving specific residues and motifs. A sequence element containing a conserved glutamic acid, called the E-box, has been described to be involved in pilus formation. Although

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