ZMS1211

Anti-Fibrin Antibody, clone 59D8 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• 别名: Fibrinogen beta chain
• 分類程式碼代碼: 12352203
• NACRES: NA.43

属性

• 生物源: mouse
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: recombinant monoclonal
• 描述: clone 59D8 ; recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 55.93 kDa; observed mol wt ~56 kDa
• 純化經由: using protein G
• 物種活性: mouse, human
• 包裝: antibody small pack of 25
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG1κ
• 表位序列: N-terminal
• Protein ID登錄號: NP_005132.2
• UniProt登錄號: P02675
• 環保替代類別: Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 基因資訊: human ... FGB(2244)

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 59D8 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Fibrin. It targets an epitope within 7 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 7 amino acids from the N-terminal region of human Fibrinogen β chain.

應用

Quality Control Testing

Evaluated by Western Blotting in Human plasma.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Fibrin in Human plasma.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Fibrin in lysates from HepG2 cells and from Mouse liver tissue..

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Fibrin in Human placenta tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fibrin in Human kidney and Human placenta tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Fibrinogen beta chain (UniProt: P02675) is encoded by the FGB gene (Gene ID: 2244) in human. Fibrinogen beta chain is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to product the mature protein that is further cleaved by thrombin to produce fibrinopeptide B (aa 31-44) and fibrinogen beta chain (45-491). Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers. Fibrin monomers spontaneously aggregate into an insoluble gel, which is then covalently stabilized by Factor XIIIa. Fibrin is shown to retain about 98% of the original covalent structure of fibrinogen. Fibrin clot can be broken down by plasmin by cleave between residues 152-153 and 163-164. A mouse monoclonal version of this clone binds to fibrin even in the presence of human fibrinogen at the concentration found in plasma. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonal. (Ref.: Litvinov, RI., and Weisel, JW (2016). Semin. Thromb. Hemost. 42(4); 333-343; Hui, KY., et al. (1983). Science. 222 (4628); 1129-1132).

外觀

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重構

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable